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Finding and Understanding Research Protocols

Learn about research protocols, their importance, and where to find them online and in books. Enhance your experiments, troubleshoot effectively, and optimize conditions for testing new ideas.

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Finding and Understanding Research Protocols

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  1. Finding and Understanding Research Protocols Angelina Hoefle The University of Texas San Antonio MBRS-RISE & MARC-U*STAR Programs 08/2006

  2. What is a protocol? • A detailed plan of a scientific or medical experiment, treatment, or procedure (Merriam Webster Online) • Indicates specific conditions, such as weight, volume, concentration, temperature, and time • Similar to a recipe

  3. Understanding the theory behind it • More efficient experiments • Makes troubleshooting easier • Helps you optimize conditions to test a different idea/set of samples

  4. Where can you find protocol books? • Current Protocols in Molecular Biology -reference section of John Peace Library (QH506.C87 1993) • Other Current Protocols titles online (Cell Bio, Immuno, Neuro, Pharmacology, Protein Science) – accessible through UTHSC computers

  5. Where can you find protocols online? • www.protocol-online.org/ • The most comprehensive free online protocol website. • Type the technique name + “theory,” “introduction,” or “background” to get more information • www.bioexplorer.net/Methods and Protocols/ • www.cellbio.com/protocols.html

  6. Where else can you find protocols online? • UTSA JPL netLibrary • www.lib.utsa.edu • Click Databases A-Z • Click “n” – find and click on netLibrary (or go directly to http://www.netlibrary.com.libweb.lib.utsa.edu/Search/BasicSearch.aspx) • Type the technique name and “method” • e.g. “immunohistochemistry method”

  7. netLibrary Books of Interest to You • Molecular Biology Problem Solver – A Laboratory Guide by Gerstein, Alan S. • Practical HPLC Method Development by Snyder, et. al. • Microscopy, Immunohistochemistry, and Antigen Retrieval Methods: For Light and Electron Microscopy by Hayat, M.A. • Electron Microscopy Methods and Protocols, by Hajibagheri, M.A.

  8. Other Online Resources • Online Books (for background information) http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=Books • Helpful Toolshttp://www.mgb.pitt.edu/moleculartoolbox.htm • Computational Resources for molecular biology http://molbio.info.nih.gov/molbio/ • Collections of books (must enter research field) http://crs.nsdl.org/collection/

  9. Acknowledgements • Dr. Gail P. Taylor • All of you!!!

  10. Questions???

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