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Speaker: Jeng-Chen Liu( 劉政成 ) Student ID: P9921003

Yeast expression of cellulase complex from rumen fungi for direct fermentation of cellulose to ethanol. Speaker: Jeng-Chen Liu( 劉政成 ) Student ID: P9921003. Graduate Institute of Bioresources, National Pingtung University of Science and Technology. Proposal for Ph. D. Program. Outline.

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Speaker: Jeng-Chen Liu( 劉政成 ) Student ID: P9921003

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  1. Yeast expression of cellulase complex from rumen fungi for direct fermentation of cellulose to ethanol Speaker: Jeng-Chen Liu(劉政成) Student ID: P9921003 Graduate Institute of Bioresources, National Pingtung University of Science and Technology Proposal for Ph. D. Program

  2. Outline • Background: fuel requirement & bioethanol • Goal: direct microbial conversion • Materials and methods: gene sources & expression • Expected results: cellulose  ethanol

  3. The problems of petroleum utilization Exhaustion Petrolic resources Global warming fossil fuels CO2+H2O

  4. The cycle of biomass energy • Renewable • Net emission(CO2)=0 Photosynthesis SugarStarchLignocellulose Lipid CO2+H2O • Ethanol-gasoline eg. E3 Biofuelseg. bioethanol

  5. Bioethanol: first generation Unauthorized cultivation Food: sugar starch bean Food price

  6. Bioethanol: second generation Unauthorized cultivation Food: sugar starch bean Food price

  7. Bioethanol: second generation Unauthorized cultivation Lignocellulose Food price

  8. The contents of lignocellulose 2 M ton * 40%=800 K ton (Prasad et al., 2007) (Murphy and McCarthy, 2005)

  9. Lignocellulose  ethanol • Pretreatment:removed lignin & hemicellulose • Hydrolysis:cellulose  glucose • Fermentation:glucose  ethanol

  10. Pretreatment Physical methodsChemical methodsBiological methods Ethanol Cellulose Hemicellulose Lignin (Lu and Mosier, 2008)

  11. Cellulose  glucose Acidic hydrolysis Enzymatic hydrolysis

  12. Conversion of cellulose to glucose Table 1. Comparison of hydrolysis methods for cellulose hydrolysis Acid hydrolysisEnzymatic hydrolysis？ Cellulose Glucose (Bungay, 2004)

  13. Lignocellulose  ethanol(SHF;SSF;DMC) • Pretreatment:removed lignin & hemicellulose • Hydrolysis:cellulose  glucose • Fermentation:glucose  ethanol

  14. Lignocellulose  ethanol(SHF) • Pretreatment • Hydrolysis: cellulase supplementation • Fermentation: microorganism Celluloseethanol

  15. Lignocellulose  ethanol(SSF) • Pretreatment • Hydrolysis: cellulase supplementation • Fermentation: microorganism Celluloseethanol

  16. Lignocellulose  ethanol(DMC) • Pretreatment • Hydrolysis: cellulase • Fermentation: microorganism Cellulose Glucose ethanol

  17. The analysis of enzyme hydrosylate and fermentation products (Chenet al., 2003) Cellulase complex (劉，2010)

  18. Glucose  ethanol • Saccharomyces cerevisiae ethanol producerethanol tolerant GRAS • Zymomonas mobilis • Escherichia coli

  19. Goal Lignocellulose  ethanol(DMC) • Pretreatment • Hydrolysis • Fermentation:S. cerevisiae Cellulose DMC Glucose ethanol

  20. Goal Lignocellulose  ethanol(DMC) • Pretreatment • Hydrolysis: cellulase • Fermentation:S. cerevisiae Cellulase complex gene from rumen fungi Cellulose DMC Glucose ethanol

  21. Rumen fungi Protocols cellulase complex genes E. coliexpression system S. cerevisiaeexpression system Biochemical characterization Cell surface display system Promoter Direct microbial conversion Bioethanol

  22. Expected results Lignocellulose  ethanol(DMC) • Pretreatment • Hydrolysis: cellulase • Fermentation:S. cerevisiae Cellulase complex gene from rumen fungi Cellulose DMC Glucose ethanol

  23. Thanks for your attention

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