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RLU (fold)

-. -. -. -. -. -. -. -. +. +. +. +. +. +. +. +. AR. AR. -. -. -. -. -. -. -. -. -. -. -. -. +. +. +. +. SRC-1. SRC-1. -. -. -. -. -. -. -. -. -. -. +. +. -. -. SRC-2. SRC-2. +. +. -. -. -. -. -. -. -. -. -. -. -. -. +. +. +. +.

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RLU (fold)

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  1. - - - - - - - - + + + + + + + + AR AR - - - - - - - - - - - - + + + + SRC-1 SRC-1 - - - - - - - - - - + + - - SRC-2 SRC-2 + + - - - - - - - - - - - - + + + + SRC-3 SRC-3 B A L-O2 Huh-7 18 100 ctrl ctrl 16 90 R1881 R1881 80 14 70 12 RLU (fold) 60 RLU (fold) 10 50 8 40 6 30 4 20 2 10 0 0 Supplementary Fig. 8. SRC-3 exhibits the highest co-activating activity to AR. SRC-1/2/3 and AR together with AR reporter were co-transfected into human L-O2 cells (A) and Huh-7 cells (B) respectively in the absence or presence of AR agonist R1881. Supplementary Figure 8

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