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Quantrain x50 Module 10 Sample Preparation

place photo here. Quantrain x50 Module 10 Sample Preparation. 3-2010. Sample Prep Basics. Specimen characteristics -volatile materials - heat sensitivity - electron beam sensitivity -electrical charging . Some general preparation rules for optimum imaging.

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Quantrain x50 Module 10 Sample Preparation

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  1. place photo here Quantrain x50Module 10Sample Preparation 3-2010 Confidential

  2. Sample Prep Basics • Specimen characteristics-volatile materials- heat sensitivity- electron beam sensitivity-electrical charging

  3. Some general preparation rules for optimum imaging • 1. Place powders or small particles directly onto a carbon coated stub or use a conductive adhesive. Move sample away from lower lens during pump down • 2. Metal or carbon coat filters before use.3. Use (conductive) silicon wafers instead of glass if a smooth surface is needed 4. Use a conductive tape to hold down samples.5. Lightly coat (about 2 nm) the sample (with a metal) :

  4. Special Importance of Mounting Media for Non-conductive Specimens • Aluminium • Carbon • Copper • Mounting adhesives • Double-sided tape • Metallic tapes • Wax or oil based mounting media

  5. Volatile Materials • Some samples become volatile under the electron beam

  6. Heat Sensitivity • The electron beam can generate heat to ‘burn’ or melt a sample

  7. Electrical Charging • Internal charge builds charge cloud - - - - - -

  8. - Metal Coating • Coating creates an electrical path to ground

  9. - Metal Coating Plasma Target molecules

  10. - Metal Coating Plasma Coating Thickness Note thin film

  11. Secure the sample- carbon paint (isopropyl based) Determine if coating is possible/necessary- conductivity is the key- low voltage may be a solution- ESEM is the solution Sample Prep Basics

  12. Mounting Media • Aluminium stub

  13. Mounting Media • Aluminium or carbon stub

  14. Mounting Media • Aluminium or carbon stub • Copper cup

  15. Mounting Media • Aluminium or carbon stub • Copper cup • Analytical mounts

  16. What to Avoid !!! • Carbon and double sided tape is not stable (for hi-res..) • Constantly expanding, absorbing, swelling, etc • Contamination of sample and chamber- finger prints- oily sputter system- has sample been in a dirty SEM?- Sitting around absorbing gas • Silver paint (blows up leaving silver particles everywhere)

  17. Mounting Methods • Adhesives

  18. Adhesives Double sided tape Mounting Methods

  19. Adhesives Double sided tape Metallic tapes Mounting Methods

  20. Adhesives Double sided tape Metallic tapes Wax or oil based to be avoided Mounting Methods

  21. Other Mounting Adhesives • Gold paint • Silver paint • Carbon paint • Colloidal carbon • Carbon tabs • Microstik

  22. Basic Preparation Techniques • FracturesMetals

  23. FracturesMetalsNon-metals Basic Preparation Techniques

  24. FracturesMetalsNon-metals SectionsHard samples- cross sections Basic Preparation Techniques

  25. Integrated Circuit Cross Section • Chemical etching

  26. Preparation Techniques • Fracturesmetalsnon-metals • Sectionshard samples- cross sections(cont..)

  27. Fracturesmetalsnon-metals Sectionshard samples- cross sections Soft samples- cross/thin sections Preparation Techniques

  28. Preparation Techniques (Cont.) • DispersionsWet

  29. Preparation Techniques (Cont.) • DispersionsWetDilution

  30. Preparation Techniques (Cont.) • DispersionsWetDilutionPipette

  31. Preparation Techniques (Cont.) • DispersionsWetDilutionPipetteAtomize

  32. Preparation Techniques (Cont.) • DispersionsWetDilutionPipetteAtomizeSandwich

  33. Preparation Techniques (Cont.) • DispersionsWetDilutionPipetteAtomizeSandwich • Dry dispersions

  34. Preparation Techniques (Cont.) • DispersionsWetDilutionPipetteAtomizeSandwich • Dry dispersionsAdhesives

  35. Preparation Techniques (Cont.) • DispersionsWetDilutionPipetteAtomizeSandwich • Dry dispersionsAdhesivesFilters

  36. Preparation Techniques (Cont.) • Live materialInsectsPlants

  37. Preparation Techniques (Cont.) • Live MaterialInsectsPlants • DryingFixationAir-drying

  38. Preparation Techniques (Cont.) • Live MaterialInsectsPlants • DryingFixationAir-dryingFreeze drying

  39. Preparation Techniques (Cont.) • Live MaterialInsectsPlants • DryingFixationAir-dryingFreeze dryingCritical Point Drying

  40. Critical Values of CPD Fluids • FLUID FORMULA CRITICAL TEMP. (°C) CRITICAL PRESSURE (Atm) • water H2O 374.0 217.7 • carbon dioxide CO2 31.1 72.9 • nitrous oxide N2O 36.5 71.7 • freon CClF3 28.9 38.2

  41. Preparation Techniques (Cont.) • ReplicasCelleous acetate sheet

  42. Preparation Techniques (Cont.) • ReplicasCelleous acetate sheetSilicone rubber (Silico-set)

  43. End of Quantrain 10 sample prep.ppt

  44. Title slide sub-title Confidential

  45. Sample Page HeaderSubhead: if used, should be sized to 24 Body copy goes here. Ut enim ad minim, quis nostrud exercit ulla ullamco. Duisete aute irure dolor in reprehenderit www.fei.com. • Bullet style: officia deserunt mollit anim id est laborum. • Bullet style: Sed ut perspiciatis de laborum veleta dolorum harum. • Bullet style: words in signature red for emphasis • Sub-bullet style: Unde omnis iste natus • Nemo enim ipsam voluptatem quia aut fugitNam libero tempore, cumin soluta nobis est placeat facere possimus, omnis voluptas ass dolor repellendus. • Bullet style: Excepteur sint occaecat cupidatat. Confidential

  46. Sample App Photo PageSubhead: if used, should be sized to 24 Caption goes here Caption goes here Confidential

  47. Sample App Photo PageDark background to be used if images are light Caption goes here Caption goes here Confidential

  48. Sample Product PageSubhead: if used, should be sized to 24 Excepteur sint occaecat cupidatat non proident, sunt in culpa qui officia deserunt mollit animid est laborum. • Sed ut perspiciatis • Unde omnis iste natus • Nemo enim ipsam voluptatem quia voluptas sis aspernatur aut fugit Confidential

  49. Color Guidelines Signature Colors Supporting Tones Accent Colors RGB 176, 26, 36 RGB 113, 113, 113 RGB 149, 130, 115 RGB 238, 88, 7 RGB 161, 161, 161 RGB 171, 151, 33 RGB 208, 208, 208 RGB 102, 125, 136 RGB 130, 160, 99 Confidential

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