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Morphology, Accuracy, and Histology of Corneal Flap Cuts Using a 200 kHz Femtosecond Laser

Morphology, Accuracy, and Histology of Corneal Flap Cuts Using a 200 kHz Femtosecond Laser. R. Khoramnia C.P. Lohmann J. Salgado C. Winkler von Mohrenfels. ASCRS 2010. Augenklinik, Klinikum rechts der Isar, Technische Universität München , Munich, Germany

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Morphology, Accuracy, and Histology of Corneal Flap Cuts Using a 200 kHz Femtosecond Laser

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  1. Morphology, Accuracy, and Histology of Corneal Flap Cuts Using a 200 kHz Femtosecond Laser R. Khoramnia C.P. Lohmann J. Salgado C. Winkler von Mohrenfels ASCRS 2010 Augenklinik, Klinikum rechts der Isar, Technische Universität München, Munich, Germany All authors have no financial interest in the subject matter of this poster. The laser system used is not FDA approved and does not yet have a CE mark.

  2. Purpose • - LASIK requires precise corneal flap cutting. • Especially the creation of thin flaps has recently gained importance for Sub-Bowman keratomileusis (SBK), which requires precise cutting. • There is a tendency towards faster femtosecond lasers that produce flaps more quickly. We analyzed flaps created with a prototype 200 kHz femtosecond laser (UltraFlap; WaveLight AG, Erlangen, Germany) concerning their cut accuracy, morphology, and histology.

  3. Material and methods Preparation 36 fresh porcine eyes: Epithelial abrasion Surgery Femtosecond laser flap cutting using the 200 kHz femtosecond laser -> Three thickness groups (100 µm, 130 µm, or 180 µm) -> Flap diameters were varied ranging from 8.0 to 9.5 mm Examination 1.) Flap diameters were measured with a sliding caliper. 2.) Flap thickness was measured with a micrometer gauge (Extramess 2000; Mahr, Esslingen, Germany). Accuracy of this device: 0.3 µm 3.) Histologic examination

  4. Results: Flap thickness Box-and-whiskerplot • Accuracy - Statistical analysis: • Significant difference between the 130- and 180-μm group (Mann-Whitney-U test; p < 0.05) • All other comparisons: No significant differences (Mann-Whitney-U test; p > 0.05). Meanvalues: 96.33±7.45 134.67±4.96 174.59±9.35 [µm]

  5. Results: Flap diameter Box-and-whiskerplot • Accuracy - Statistical analysis: • The deviation between measured and attempted diameters was not significantly different between the four diameters (Kruskal-Wallis test; p > 0.05). Meanvalues: 8.03±0.15 8.03±0.15 9.09±0.10 9.54±0.15 mm [mm]

  6. Results: Histological examination • (A) A 180-µm flap in the region of the side cut. • (B) Stromal bed in the region of the side cut (flap thickness: 100 µm). • A 130-µm flap and the stromal bed. • H&E staining; original magnification, x20. Histology showed a smooth cut with very little to almost no changes in the corneal structure.

  7. Conclusions • Flap creation was performed easily without any complications. • Morphology and accuracy of the cuts were very reliable and precise. • Histology showed a smooth cut with very little to almost no changes in the corneal structure. • -> Therefore, the 200 kHz femtosecond laser seems to be a promising new device in refractive surgery.

  8. CONTACT Klinik und Poliklinik für Augenheilkunde Klinik und Poliklinik für Augenheilkunde am Klinikum rechts der Isar Ismaninger Str. 22 81675 Munich GERMANY SECRETARIAT Tel. + 49 89 41 40-23 20 Fax + 49 89 41 40-48 58 E-Mail: augenklinik@lrz.tum.de Internet: www.augenklinik-mri.de 8

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