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Quality Of Lamellar Dissections Prepared With The 500 Khz Femtosecond Laser System

Quality Of Lamellar Dissections Prepared With The 500 Khz Femtosecond Laser System. Volkan Hurmeric, MD, Sonia H. Yoo, MD, Mohamed Abou Shousha, MD, William W. Culbertson, MD, Noel Ziebarth, PhD, Jean-Marie Parel, PhD. Bascom Palmer Eye Institude Miami-FLorida. Financial Disclosure :

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Quality Of Lamellar Dissections Prepared With The 500 Khz Femtosecond Laser System

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  1. Quality Of LamellarDissectionsPreparedWithThe 500 Khz Femtosecond LaserSystem Volkan Hurmeric, MD, Sonia H. Yoo, MD, Mohamed Abou Shousha, MD, William W. Culbertson, MD, Noel Ziebarth, PhD, Jean-Marie Parel, PhD. Bascom Palmer Eye Institude Miami-FLorida FinancialDisclosure: Sonia H. Yoo and William W. Culbertsonareon thespeakers bureaufor Carl ZeissMeditec

  2. Quality of Lamellar Dissections Prepared with the 500 Khz Femtosecond Laser System • Purpose: • To study the quality of lamellar dissection prepared with the 500 kHz femtosecond laser (FL) • Materials and Methods: • 6 human globes were cut with the VisuMax 500 kHz FL. • 200, 300, and 400 micron deep cuts were made each at low (180 nJ) and high (340 nJ) energy settings [1.6 µ spot and line sep]. • Environmental scanning electron microscopy (eSEM) and atomic force microscopy (AFM) were performed on the tissue to assess the quality of the cuts.

  3. Results Cut depth : 200 µm Energy : 180 nJ Cut depth : 200 µm Energy : 340 nJ eSEM • The 200 micron cut with high energy settings resulted in smooth dissections.

  4. Results Cut depth : 200 µm Energy : 180 nJ AFM Cut depth : 200 µm Energy : 340 nJ

  5. Results Cut depth : 300 µm Energy : 180 nJ Cut depth : 300 µm Energy : 340 nJ eSEM • The 300 micron cut with low energy settings resulted in the smoothest dissection.

  6. Results Cut depth : 300 µm Energy : 180 nJ AFM Cut depth : 300 µm Energy : 340 nJ

  7. Results Cut depth : 400 µm Energy : 180 nJ Cut depth : 400 µm Energy : 340 nJ eSEM • The 400 micron cut with low energy settings resulted in the roughest dissection.

  8. Results Cut depth : 400 µm Energy : 180 nJ AFM Cut depth : 400 µm Energy : 340 nJ

  9. Roughness from AFM scans Low Energy High Energy

  10. Conclusions • 200 and 300 micron lamellar cuts with the 500 kHz VisuMax FL result in excellent quality dissections. • “High frequency” roughness is lowest for the 300µm scans (both low and high energy).  • Depth of cut and laser energy settings seem to affect the quality of the dissection.

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