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B. C. A. Recipient class I. CD31. merge. F. D. E. merge. Donor class I. CD31. Supplemental Digital Content 1- Figure 1.
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B C A Recipient class I CD31 merge F D E merge Donor class I CD31 Supplemental Digital Content 1- Figure 1. Dual colour confocal microscopy illustrates donor origin of EC, 8 weeks after transplantation. PVG.RT1u hearts, 8 weeks after allotransplantation, stained with MN4-91-6 (green, against RT1Aa, recipient MHC class I) detects infiltrating cells (A), stained for CD31 (red) to detect EC (B), merging A and B shows no co-localisation. Panel D was stained with U9F4 (green, against RT1Au, donor MHC class I), or with MoAb to CD31 to detect EC (E), merging shows co-localisation, yellow, of donor MHC class I with CD31 (F).