Making our own digestol pill digest-all

1. By: Ayla Norris and Zhou Li 2009 Skills for Success Workshop Other Enzyme Ingredients Introduction Digestol is a dietary supplement by KramerLabs that is designed to provide the enzymes necessary to digest starches, fats, proteins, and dairy products. It is claimed to relieve digestive problems. We investigated the enzymes present in Digestol and attempted to identify the best candidate sources of enzymes to use if we made our own dietary suppliment like Digestol. Human gastric lipase Human bile-salt activated lipase Human pancreatic lipase Bacteroidesfragilis Beta-galactosidase Pineapple papain Above: human pancreatic amylase. Right: Rice amylase. Conserved regions might indicate active sites or starch-binding regions It is difficult to determine whether human amylases or amylases specific to major sources of starch like the rice amylase would be more effective, because the physiological conditions are different between humans and rice. Making our own digestol pilldigest-all Human salivary amylase References: http://www.pdb.org/pdb/home/home.do http://www.kramerlabs.com/index.asp?template=digestol Gary D. Brayer et al. Subsite Mapping of the Human Pancreatic α-Amylase Active Site through Structural, Kinetic, and Mutagenesis Techniques. Biochemistry, 2000, 39 (16), pp 4778–4791 Acknowledgements: NIH and PEER ISMD Award # 1R25GM086761-01