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Making Sense of our Restriction Digests

Making Sense of our Restriction Digests. Or not…. Theoretical Pvu II digests of plasmids. p. 2B-10. Why are there 3 bands in the uncut lane?. p. 2B-10. What is the size of the insert?. 2.9 Kb. 700 bp - 700 bp = 0 bp. p. 2B-13. Is there a Pvu II site in the insert

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Making Sense of our Restriction Digests

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  1. Making Sense of our Restriction Digests Or not…

  2. Theoretical PvuII digests of plasmids p. 2B-10

  3. Why are there 3 bands in the uncut lane? p. 2B-10

  4. What is the size of the insert? 2.9 Kb 700 bp - 700 bp = 0 bp p. 2B-13

  5. Is there a PvuII site in the insert Are there multiple bands? p. 2B-13

  6. Do the results from the digest and the PCR agree? 700 bp - 700 bp = 0 bp 200 bp - 200 bp = 0 bp p. 2B-14

  7. How do we figure out the insert size from the 20JM4.10 digest? 2900 bp 1200 bp 400 bp 1200 bp + 400 bp-700 bp = 900 bp

  8. Section 12 ResDig Gel Run 9/28

  9. Section 34 ResDig Gel

  10. Section 58 Res Digest gel

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