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HAEMOGLOBIN ESTIMATION

HAEMOGLOBIN ESTIMATION. Sahli’s method. Aim. To estimate the Haemoglobin levels in mg/dl using Sahli’s method. Equipment. Sahli’s haemoglobinometer with tube, pipette, colour comparator and stirrer Hydrochloric acid, 0.1N Distilled water Blood sample. SAHLI’S HAEMOGLOBINOMETER.

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HAEMOGLOBIN ESTIMATION

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  1. HAEMOGLOBIN ESTIMATION Sahli’s method

  2. Aim • To estimate the Haemoglobin levels in mg/dl using Sahli’s method

  3. Equipment • Sahli’s haemoglobinometer with tube, pipette, colour comparator and stirrer • Hydrochloric acid, 0.1N • Distilled water • Blood sample

  4. SAHLI’S HAEMOGLOBINOMETER Haemoglobinometer tube 20 μl mark Mark 2 Stirrer Comparator Haemoglobinometer pipette

  5. PROCEDURE • Fill N/10 HCl up to mark 2 of the tube • Pipette out 20 μL of blood, wipe tip with cotton to remove excess blood • Introduce blood into HCl in the tube • Mix, keep for 10 minutes • Add distilled water, stir, compare colour in comparator • Continue adding water and mixing until colour matches • Read lower meniscus on tube for Hb in g/dL

  6. Fill N/10 HCl up to mark 2 of the tube Mark 2 N/10 HCl

  7. Pipette out 20 μL of blood 20 μL mark Blood sample Wipe excess blood around tip with cotton

  8. Introduce blood into tube N/10 HCl

  9. Mix, keep for 10 minutes

  10. Add distilled water, stir

  11. Continue adding, stirring until colour matches with standard

  12. Read Hb from lower meniscus, express as g/dl Read Hb level

  13. QUESTIONS • Describe the procedure • What is the principle behind the method? • What are the advantages & the disadvantages of this method? • What other methods are available? • Which method is considered ideal? • What is the reference haemoglobin range? • What is the importance of haemoglobin estimation?

  14. MORE RELATED QUESTIONS • Define anemia / polycythemia • Classify anemia. • Name some causes for anemia / polycythemia • What is Drabkin’s solution? ____________

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