Structures of the Purine and Pyrimidine Bases

Structures of the Purine and Pyrimidine Bases

Example of the Structure of a Nucleotide Base Nucleoside Nucleotide

Chemical Structure of a Nucleic Acid Voet Fig. 3-6

Double Helical Structure of DNA Note that the strands run antiparallel Note that the helix is 20 Å wide. There are about 10 base pairs per turn of the helix. One turn of the helix is 34 Å The base pairs are 3.4 Å apart Major groove Voet Fig. 3-9

Base Pairing Voet Fig. 23-1

Many proteins associate With DNA through the Major and Minor grooves Major Groove http://www.clunet.edu/BioDev/omm/gallery.htm

Space-filling Model of B-DNA Backbones of the strands are bright green and bright red The bases on the corresponding backbones are lighter green and pink

Helical Structures A-DNA B-DNA Z-DNA Voet Fig. 23-2

Supercoiling of DNA Supercoiled DNA Relaxed DNA

Denaturation of DNA Voet Fig. 23-15

Restriction Endonucleases Voet Fig. 3-18

Restriction Digest Cutting DNA with Restriction Endonucleases followed by Analysis by Gel Electrophoresis B A C A C B

Visualizing DNA Fragments in Gels Voet Fig. 3-20

Southern Analysis Voet Fig. 23-28

Chain Termination Method of DNA Sequencing Voet Fig. 3-23

DNA Sequencing Instruments Fluorescent dye-conjugated derivatives of the Dideoxynucleotides can be used in DNA Sequencing Instruments

Automated Sequencing Example of data obtained from a DNA sequencing reaction using fluorescent (dye-conjugated) derivatives of dideoxynucleotides Voet Fig. 3-25

Polymerase Chain Reaction Amplification of DNA Garrett and Grisham Fig. 13-21 See Voet Fig. 3-32

Plasmids can Serve as Cloning Vehicles (Vectors) Voet Fig. 3-27

Construction of a Recombinant DNA Voet Fig. 3-29

Selection of Transformants Vector DNAs must be capable of replicating in the host cells and must carry a selectable marker

Selection of Transformants of Interest Voet Fig. 3-31

Structures of the Purine and Pyrimidine Bases