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Pet Allergen Diagnosis Protein Array

Pet Allergen Diagnosis Protein Array. Excelsior Bio-system Kang Hsin Chen. What is Allergic Reaction?. Over reaction to common environment substance Mite 、 pollen 、 hair 、 fungi Soybean 、 wheat 、 milk 、 pork etal. What Causes for Allergies?. Type I acute anaphylactic reaction

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Pet Allergen Diagnosis Protein Array

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  1. Pet Allergen DiagnosisProtein Array Excelsior Bio-system Kang Hsin Chen

  2. What is Allergic Reaction? • Over reaction to common environment substance • Mite、pollen、hair、fungi • Soybean、wheat、milk、pork etal.

  3. What Causes for Allergies? • Type I acute anaphylactic reaction • IgE - degranulation of Mast cell • Mediator released • Atopy、Dermatitis、Asthma

  4. IgE - Degranulation of Mast Cells Cell-bound form IgE

  5. Mediator Released

  6. Atopic Canine • Atopic Dermatitis (AD) • inhalent or GI-intake? • Age of the onset: 1-3 years • Clinical presentation: pruritus、erythema、chewing、licking、salivary staining

  7. Clinical presentation

  8. Health Care of Atopic Pet • Rule out other disease • Avoid second infection • Pet’s serum specific IgE profile • Food design based on allergen result • immunotherapy

  9. Traditional Diagnosis • Clinical signs and history • Skin test • Serological test • ELISA/ • RAST(radioallergosorbent test)

  10. What defect about skin test? • Shaved the hair • Dangerous to pet • Food allergen unable • 8-10 allergen could be tested at one time

  11. What defect about traditional serological test? • Too many false negative results • Sensitivity is not enough • Too many false positive results • Specificity is not enough • Score is confused • Signal range is too small

  12. Pet Allergen Panel in Taiwan

  13. Our Total Solution • Immunostrip • Allergen Array • Health Food • Probiotic • Balance TH1/TH2 • Low allergic Food • Formula based on test result

  14. Immunostrip • Rule out other possibility • First line screening • Fast and Easy

  15. Immunostrip

  16. Technology- Allergen Preparation • More than 200 items of allergens purified • Covering a variety range of food and air-borne allergens, including dust mites, animal hairs, pollens, Chinese herbals, meat and vegetables etc. • Whole proteins extracted by customized rapid buffer extraction and size exclusion processes according to different types of raw material • Quality control and assurance established • SDS-PAGE analyzed for batch to batch control • Major allergen items were tested and confirmed by clinical samples (both human and canine)

  17. mite pollen fungi Food-1 Food-2

  18. Fluorescence Allergen Array • Include 62 allergic items • Design for Taiwan area • Provide the specific IgE profile

  19. Principle of Allergic Array Array Spotter

  20. Principle of Allergic Array

  21. Principle of Allergic Array Laser Scanner

  22. Example of Allergen Array Results Hyposensitivity Hypersensitivity

  23. Our 62 Panel Set • Mite • Blomia tropicals stoirge mite • Dermatophagoides pteronyssinus • Dermatophagoides farinae • Acarus Siro • Tyrophagus Putrescentiae

  24. Our 62 Panel Set • Insect and others • Tsutsugamushi mite • Tick • Flea • Aedes Communis • Cules Pipiens • Blatella Germanica

  25. Our 62 Panel Set • Dander and Skin scrape • Columba Palumbus • Human Dander • Cow hair and skin scrape • Sheep hair and skin scrape • Cat hair

  26. Our 62 Panel Set • Fungus • Cladosporium Herbharum • Candida albicans • Alternaria alternate • Penicillium notatum • Aspergillus fumigatus

  27. Our 62 Panel Set • Pollen • Eucalyptus globules • Poa Pratensis • Amaranthus retroflexus • Rumex Acetosella • Ambrosia Artemisii folia(elatior) • Acacia Baileyana • Cynodon Dactylon

  28. Our 62 Panel Set • Foods • Milk、egg white、egg yolk、cheese • Duck、lamb、beef、chicken、turkey、pork • Soybean、peanut、rice、wheat、carrot、corn、patatoe • crab、shrimp、mackerel、sardine、anchovy、cod fish、salmon • watermelon、mango、cantaloupe、pear、mandarine、banana、apple • Yeast baker's、yeast brewer's • Other • Flax

  29. Our Results • From 2007/1-2007/6 • 183 Pets (canine) • 81% canine have allergic reaction • 4 different score • No reaction/minor/medium/serious

  30. Top 10 allergens Inhalant GI-intake

  31. Summary Benefits of array based allergens test

  32. Benefits of array based allergens test • No more need for sedation • No more need for shave the pets • No more need a lot of sera • Item is flexible for different area or customer need

  33. Benefits of array based allergens test • We can test food allergens • We can economize your time and money • You can get pet IgE profile at one test • You can list of foods that the pet should avoid

  34. Thank You!

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