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PHAGOCYTIC ACTIVITY OF EARTHWORM COELOMYCYTES USED AS A SUBLETHAL BIOMARKER OF METAL TOXICITY

PHAGOCYTIC ACTIVITY OF EARTHWORM COELOMYCYTES USED AS A SUBLETHAL BIOMARKER OF METAL TOXICITY. Sauvé S, Hendawi M, Brousseau P, Fournier M Département de chimie – Université de Montréal INRS-Institut Armand Frappier-Santé Humaine. Earthworms.

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PHAGOCYTIC ACTIVITY OF EARTHWORM COELOMYCYTES USED AS A SUBLETHAL BIOMARKER OF METAL TOXICITY

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  1. PHAGOCYTIC ACTIVITY OF EARTHWORM COELOMYCYTES USED AS ASUBLETHAL BIOMARKER OF METAL TOXICITY Sauvé S, Hendawi M, Brousseau P, Fournier M Département de chimie – Université de Montréal INRS-Institut Armand Frappier-Santé Humaine

  2. Earthworms • Earthworms lead an essential ecological role for maintaining soil health, fertility, structure, nutrient cycling, … • Standard worm assays evaluate lethality • Sublethal markers such as reproduction and immunocompetence can be quantified

  3. Worm species • Terrestrial earthworms • Lumbricus terrestris • Eisenia fetida • Aporrectodea turgida • Aquatic worm • Tubifex tubifex

  4. Cell Extrusion • By puncture with pasteur pipette or syringe • Ethanol extrusion • Electrical stimulation • Ultrasound • Extruded cells are suspended in a Lumbricus Basal Salt Solution (LBSS)

  5. Species-specific parameters

  6. Phagocytosis Earthworm coelomocytes (Eisenia fetida)

  7. Flow Cytometry

  8. Phagocytosis

  9. Silver and Arsenic

  10. Cadmium and Copper

  11. Mercury

  12. Lead and Zinc

  13. In vitro Toxicity thresholds ND = not detected * = extrapolated

  14. Transfer in vitro to in vivo • In vitro cellular exposure • Petri dish, solution exposure • Soil incubation (using the OECD reproduction protocol) • Field, in situ exposure in mesocosm « worm bags » • Some recent field applications to the evaluation of cement factory emissions

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