Advanced Topics in Micro-Batch Crystallization

1. Advanced Topics in Micro-Batch Crystallization Ran Meged 1

2. Advanced Topics in Micro-Batch Crystallization * BasicWASPRUN and the interpreter WASP (Windows Automatic Sample Preparation) Scripts to extend screening capabilities Pick and Mix * * 2

3. WASPRUN General Description WASPRun is a program to run screening experiments. It provides a user interface and supplies parameters to the WASP liquid handling script interpreter. 3

4. WASPRUN General Description WASPRun is a program to run screening experiments. It provides a user interface and supplies parameters to the WASP liquid handling script interpreter. filename.xpp 4

5. WASPRUN General Description WASPRun is a program to run screening experiments. It provides a user interface and supplies parameters to the WASP liquid handling script interpreter. filename.xpp Passing all parameters required by the experiment in an appropriately named Include file (.XPH). And launch WASP, 5

6. ? What is WASP WASP is a software package to control Douglas Instruments syringe drives and plate loaders. WASP provides a means for moving and dispensing small quantities of liquid over an array of wells, in a simply programmed but flexible way. ? How does WASP work The principal function of WASP is to read a list of instructions provided by you, the user, and turn these instructions into movements of the syringe drives and plate loader. 6

7. ? What is WASP WASP is a software package to control Douglas Instruments syringe drives and plate loaders. WASP provides a means for moving and dispensing small quantities of liquid over an array of wells, in a simply programmed but flexible way. ? How does WASP work The principal function of WASP is to read a list of instructions provided by you, the user, and turn these instructions into movements of the syringe drives and plate loader. Interpreter that get a set of Definitions and scripts commands and execute them… 7

8. File Relationships Configuration Files User Files Programs Script Files WASPRUN.EXE * . XPP * . XPH * . XPT DEFAULTS.XPH [ PLATE*.XPH] WASP.EXE SYRINGES.DAT PLATES({Oryx|IMPAX}).DAT HARDWARE.CFG Into Front Panel and Robot 8

9. File Relationships Configuration Files User Files Programs Script Files WASPRUN.EXE * . XPP * . XPH * . XPT DEFAULTS.XPH [ PLATE*.XPH] WASP.EXE SYRINGES.DAT PLATES({Oryx|IMPAX}).DAT HARDWARE.CFG 9

10. File Relationships Configuration Files User Files Programs Script Files WASPRUN.EXE * . XPP * . XPH * . XPT DEFAULTS.XPH [ PLATE*.XPH] WASP.EXE SYRINGES.DAT PLATES({Oryx|IMPAX}).DAT HARDWARE.CFG 10

11. File Relationships Configuration Files User Files Programs Script Files WASPRUN.EXE * . XPP * . XPH * . XPT DEFAULTS.XPH [ PLATE*.XPH] SYRINGES.DAT 11

12. File Relationships Configuration Files User Files Programs Script Files WASPRUN.EXE * . XPP * . XPH * . XPT DEFAULTS.XPH [ PLATE*.XPH] WASP.EXE SYRINGES.DAT PLATES({Oryx|IMPAX}).DAT HARDWARE.CFG Into Front Panel and Robot 12

13. File Relationships Configuration Files User Files Programs WASPRUN.EXE * . XPP * . XPH * . XPT DEFAULTS.XPH [ PLATE*.XPH] WASP.EXE SYRINGES.DAT HARDWARE.CFG Into Front Panel and Robot 13

14. In practice, your list of instructions will be contained in a text file which is the `experiment' file, and carries a ".XPT" extension. An experiment file is created with a suitable text editor. WASP is then invoked to run the experiment. 14

15. CommandParameters Air volume using line-name Array array-nameishsizexvsizestarting ath,von platepnum [ with{HORIZONTAL|VERTICAL} {SERPENTINE|WRAPAROUND}scanning ] ; Any text to end of line ............. Dispense volume to array-name using line-name [ ,volume using line-name [ ,...] ] [ waitingdelay ] Else End End Sequence Endif If expression Include <file name> Load volume using line-name [ ,volume using line-name [ ,...] ] [ waitingdelay ] OilDrop volume on array-name using line-name Pause Any text to end of line ............. Platepnum is plate-name Prompt Any text to end of line ............. Report Any text to end of line ............. Reposition Rinse volume in array-name [ ,array2 [ ,array3 ] ] using line-name [ , volume using line-name [ ,...] ] [ waitingdelay ] Sequence num-wells Set Flowof line-name to flow-rateul/s Set array-name parameter TOvalue Shell DOS Command Text ....... Syringesdnum is syringe-namecalled line-name [on axis {Z | V}] Transfer r-volume from r-array-name to d-array-name using r-line-name mixing with \ p-volume using p-line-name Use Well array-name m, n ... WellCount()... function returning index of current sequence loop ... WellCol(array)... function returning column being currently addressed in specified array ... WellRow(array).. function returning row being currently addressed in specified array Withdraw volume from array-name using line-name [ , volume using line-name [ ,...] ] [ waitingdelay ] 15

16. Selectable screen on Double Plate 16

17. Selectable screen on Double Plate 17

18. Greiner Stock Plate to (selectable screens) on 4 VB Plates 18

19. Greiner Stock Plate to (selectable screens) on 4 VB Plates 19

20. 3 Sub-well Routine to QrystalQuick Greiner plate 20

21. 21

22. 22

23. 23

24. 24

25. 25

26. 26

27. 27

28. * 28

29. 29

30. 30

31. Thank you. 31

32. CRYSTOOL 4.1 http://porter.llnl.gov/crystool4.1 32

33. CRYSTOOL 4.1 33

34. CRYSTOOL 4.1 34

35. CRYSTOOL 4.1 35

36. CRYSTOOL 4.1 36

37. CRYSTOOL 4.1 37

38. CRYSTOOL 4.1 38

39. CRYSTOOL 4.1 39

40. CRYSTOOL 4.1 http://www-structure.llnl.gov/Xray/tutorial/High_Throughput_EMBL_full.htm 40

41. Thank you again. 41