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Development and characterization of the common bean diversity panel (ADP)

Development and characterization of the common bean diversity panel (ADP). BREEDING LOCALLY-ADAPTED PULSE CROPS FOR ENHANCED YIELD AND SEED QUALITIES: AN INTEGRATED, OUTCOME-BASED PLAN FOR ARS. Karen Cichy ARS East Lansing, MI Mike Grusak ARS Houston, TX Phil Miklas ARS Prosser, WA

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Development and characterization of the common bean diversity panel (ADP)

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  1. Development and characterization of the common bean diversity panel (ADP)

  2. BREEDING LOCALLY-ADAPTED PULSE CROPS FOR ENHANCED YIELD AND SEED QUALITIES: AN INTEGRATED, OUTCOME-BASED PLAN FOR ARS • Karen Cichy ARS East Lansing, MI • Mike Grusak ARS Houston, TX • Phil Miklas ARS Prosser, WA • Talo Pastor-Corrales ARS Beltsville, MD • Tim Porch ARS Mayaguez, PR James Beaver UPR Mayaguez, PR Consuelo Estevez UPR Mayaguez, PR Gail Wisler ARS-NPL Roy Scott ARS-NPL

  3. The main goal of the project is to increase common bean production in Sub-Saharan Africa, through the development of germplasm resources, molecular tools, and scientific knowledge.

  4. OBJECTIVES 1. Develop an Andean Diversity Panel (ADP) comprised of 300-500 dry bean lines for genetic characterization of targeted traits and identi- fication of breeding materials. 2. Identify and develop Andean germplasm with improved biological nitrogen fixation (BNF) and improved production on degraded low input soils. 3. Improve tolerance to abiotic stresses-drought and heat. 4. Identify Andean common bean cultivars with disease resistance genes for the control of the most widespread and yield-reducing diseases in target countries.

  5. OBJECTIVES 5. Determine the phenotypic variation of seed phytic acid , flavonoids, and cooking time. 6. Determine the phenotypic variation and genetic control of seed mineral concentrations, pod mineral partitioning, and pod dry matter partitioning in Andean beans.

  6. Objective 1: Develop Andean Diversity Panel

  7. Increase of the ADP Panel Subsets Increased in: Prosser, Washington Isabela, Puerto Rico South Africa

  8. Objective 2: Identification of ADP germplasm efficient under low fertility conditions

  9. Morogoro, Tanzania

  10. Trial of 95 landraces in Tanzania, 2012Results from top 6 lines Correlation 0.47**

  11. Objective 3: Evaluate Abiotic Stress Response Top 25 performers for Drought – Othello, WA 2012 Drought Intensity Index = 40% Yield (kg ha-1Geomean)

  12. Objective 4: Identify disease resistant germplasm within the ADP 1-9 Scale, CBB Blue- 3353 Red- 484A Lines Limited Common bacterial blight (CBB) Resistance in ADP Correlation coefficient between strains: 0.92

  13. Response to strains 484A and 3353 of CBB285 lines; 7 R., 16 Int., 262 S.

  14. ADP Response to NL3 Strain of BCMNVOut of 303 lines: 37 with I gene and 3 with bc3 gene

  15. Ashy stem blight responseusing Band-aid method

  16. Objective 5: Determine the phenotypic variation of seed phytic acid, flavonoids, and cooking time Cooking Time on 100 ADP lines

  17. Objective 6: Determine the phenotypic variation and genetic control of seed mineral concentrations Elemental analysis of 40 Andean Entries – Seeds Potential impact: Broad diversity exists for seed mineral concentrations. Association mapping with the ADP should lead to the identification of seed mineral QTLs for breeders.

  18. Diversity Analysis- Based on 73 informative SNPs ADP Currently in the process of being SNP genotyped with 6,000 BeanCAP SNPs for AM Analysis

  19. Sharing of ADP population Development of Access Database

  20. Concluding Comments • Initial findings in ADP • Limited disease resistance • Promising efficiency under low fertility, and for BNF • Useful range of phytonutrient concentration and cooking times • ADP dataset will be important: • For mapping new traits/developing markers in Andeans • Establishing an initial baseline of African germplasm in research and breeding efforts • Determining research/breeding priorities focused on Africa Acknowledgements:

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