120 likes | 229 Views
Figure 1. Development of golden mosaic disease in the common bean lines engineered for resistance to Bean golden mosaic virus mediated RNAi (T), compared to non-transgenic varieties (NT). Two transgenic lines presented similar resistance curves. Plants were
E N D
Figure 1. Development of golden mosaic disease in the common bean lines engineered for resistance to Bean golden mosaic virus mediated RNAi (T), compared to non-transgenic varieties (NT). Two transgenic lines presented similar resistance curves. Plants were cultivated in the field in 2007 and 2008. Disease evaluation was done visually by recording the first date of vein clearing.
Rice Tungro Disease From IRRI
Resistance to Cassava Viruses for Africa Resistance against cassava Geminiviruses via the ‘G5 protein and siRNAs; CP siRNA for a potyvirus (cassava brown streak) G5 CsVMV promoter 35S promoter protein siRNA siRNA siRNA against ACMV & EACMV against CBSV against EACMV against ACMV protein -mediated PTGS -mediated
Cultivars selected based on farmer input in West and East Africa • To date 8 farmer preferred cultivars produce transgenic tissues • 5 cultivars yielded transgenic plantlets (to date), examples below: Progress in developing transgenic plants of farmer-preferred cassava cultivars Transgenic plant of Ugandan cv. Ebwanateraka Transgenic plant of Nigerian cv. TME117 GFP expressing roots GFP expressing roots & leaves
Cassava Field Trials Kenya • The VIRCA confined field test (CFT) application is currently at the IBC stage. • This is being split into two applications. One for each technical approach. • The BioCassava Plus (BC+) CFT is currently at the IBC stage. • The second version of the application was resubmitted to the IBC in March. • Uganda • The VIRCA CFT was approved by the NBC in January. • The import permit was applied for in early April and is expected during May. • Best case scenario is planting in August.
Uganda, NaCRRI Infrastructure Improvements CFT Site Biosafety Level II Screenhouse Tissue Culture Facility
Advances in Controlling Insects: Beyond Cotton and Maize Insect resistant cowpea Insect resistant brinjal/eggplant Insect resistant potato
Most popular vegetable in India after potato Meaty texture, so staple in vegetarian diets 25 calories per serving, with no fat! Common Vegetable Bt brinjal
Grown in all vegetable-growing zones in India but prominently in 8 States: Andhra Pradesh, Bihar, Karnataka, Maharashtra, Orissa, Tamil Nadu, Uttar Pradesh and West Bengal
25 - 80 rounds of pesticides sprayed on brinjal crop during each growing season
IIVR Varanasi IIVR Varanasi
Benefits to farmers Bt Non-Bt Non-Bt Cash benefit of Rs 16,000-19,000 per acre and Rs 2,000 crore to our nation