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Inducing Mutations in Paramecia with Ultraviolet Light

Inducing Mutations in Paramecia with Ultraviolet Light. Megamecia. Introduction. Model Organism Paramecium Tetraurelia Mutagen Ultraviolet Radiation. Paramecium Tetraurelia. Ultraviolet Radiation. Electromagnetic Spectrum 100 nm – 400 nm range

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Inducing Mutations in Paramecia with Ultraviolet Light

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  1. Inducing Mutations in Paramecia with Ultraviolet Light Megamecia

  2. Introduction • Model Organism • Paramecium Tetraurelia • Mutagen • Ultraviolet Radiation

  3. Paramecium Tetraurelia

  4. Ultraviolet Radiation • Electromagnetic Spectrum • 100 nm – 400 nm range • Causes pyrimadine dimers to form in DNA (Goodsell, 2001) • Pyrimadine dimers may result in the formation of a point mutation. http://www.uvsciences.com/uvtech.html

  5. Pyrimadine Dimers • Covalent bond between two adjacent pyrimadine bases (Thyamine, Cytosine) • Point Mutation – Transition Mutation • Switching of complementary base pairs A-T becomes G-C (Russell, 2010) http://www.mun.ca/biology/scarr/F16-20,25.jpg http://www.uvsciences.com/images/dna.jpg

  6. Methods • 500 μL culture samples added to tissue culture tray • Samples exposed to UV radiation in a UV chamber. • Exposed between 10 and 60 minutes in 10 minute intervals

  7. Methods • Culture trays wrapped in tin foil immediately after exposure. • Paramecia have the ability to repair DNA while exposed to white light • Repeated 30 minute exposure with new samples • 200 μL klebsiella inoculated wheat media added to each sample after exposure • Samples checked periodically for changes • Photos taken using a digitial microscope and Applied Vision • Measurements done with Image J

  8. RESULTS • 30 minutes was the optimum time to keep the cells in UV light • Mutant cells were 22% larger in length & 15% larger in width

  9. RESULTS MUTANTS CONTROL

  10. DISCUSSION Other mutations that weren’t able to be cultured

  11. DISCUSSION • Experiment could be taken further • Analyze DNA to pinpoint where mutation occurred • Experimental errors • Tissue culture trays should have been checked for cells before exposure to Ultraviolet radiation • Ultraviolet radiation successfully caused a mutation

  12. REFERENCES Goodsell, D.S. (2001). The Molecular Perspective: Ultraviolet Light and Pyrimidine Dimers. The Oncologist, 6(3), 298-299. Retrieved from http://theoncologist.alphamedpress.org/cgi/content/full/6/3/298 Russell, P.J. (2010) iGenetics: A Molecular Approach 3rd Ed. San Francisco, CA: Pearson Education, Inc.

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