1 / 19

A Summary By William Dotson and Danny Rose

Pattern of Polymorphism After Strong Artificial Selection in a Domestication Event Hidenki Innan and Yuseob Kim. A Summary By William Dotson and Danny Rose. Outline of the Presentation. Background Information Experimental Methods and Results Discussion and Implications.

Download Presentation

A Summary By William Dotson and Danny Rose

An Image/Link below is provided (as is) to download presentation Download Policy: Content on the Website is provided to you AS IS for your information and personal use and may not be sold / licensed / shared on other websites without getting consent from its author. Content is provided to you AS IS for your information and personal use only. Download presentation by click this link. While downloading, if for some reason you are not able to download a presentation, the publisher may have deleted the file from their server. During download, if you can't get a presentation, the file might be deleted by the publisher.

E N D

Presentation Transcript


  1. Pattern of Polymorphism After Strong Artificial Selection in a Domestication EventHidenki Innan and Yuseob Kim A Summary By William Dotson and Danny Rose

  2. Outline of the Presentation • Background Information • Experimental Methods and Results • Discussion and Implications

  3. Background Information Study Objective • Determine a model for the process of strong artificial selection during a domestication event. • Artificial Selection • Differs from advantageous mutant selection • i.e. acts on previously neutral allele* • Domestication Events • Causes fixation of a predetermined advantageous allele • i.e. dogs, cows, barley, etc.

  4. Background Information Cont. Applications Find domestication genes primarily in crops such as corn and rice • Future crop improvements • Disease models • Greater crop yields

  5. Experimental Theory Linkage Disequilibrium Equilibrium – the genotype of a chromosome at one locus is independent of its genotype at the other locus • Disequilibrium- there is a nonrandom association between a chromosome’s genotype at one region and its genotype at the other region • Selection* • Genetic drift • Population admixture • Can be calculated as a numerical value.

  6. Experimental Theory Selection and Linkage Disequilibrium • Artificial selection puts unequal pressure on a what was likely previously a neutral allele • When the allele is selected for, it carries a random selection of surrounding genes with it • Genotypes from region to region in each generation are no longer independent of each other • These quantifiable effects are used as signatures for selected genes • Example – young allele at high frequency

  7. Experimental Methods • Measurements of Artificial Selection • Polymorphism • 3 measures of polymorphism in this study - qS variation in segregating site -qpvariation of pairwise nucleotide differences -qHhomozygosity of the derived allele per site • Low amounts of polymorphism suggest the influence of selection (signature) • Using history of frequencies of the allele classes, a model and simulation were developed

  8. Experimental Methods Simulation of a Domestication Event – Bottleneck Current Population N1 Ancestral Population N2 Neutral Allele in Wild Progenitor Population (Genetic Drift) Subset Founder Population and Artificial Selection Begins (td)

  9. Experimental Methods • Basis model used for experimental simulations to investigate patterns of DNA polymorphism after domestication with and without selection.

  10. Simulation 1 Polymorphism with Selection • Constant population size • 5000 Replications • Polymorphism is represented as qp, which is ideally equal to 4Nmin a constant size population • Several initial frequencies were studied and compared with the standard selective sweep model • Different strengths of selection were compared in the second figure.

  11. Simulation 1 Standard Selective Sweep Model

  12. Simulation 2 • Two simulations were used to determine the joint effects of selection and population bottleneck • The severity of the bottleneck differs in each case. • The level of polymorphism is reduced by the bottleneck regardless of the effect of selection • The qualitative effect of p is almost identical in both models

  13. Simulation 2

  14. Simulation 3 Measured the effects of • different values for initial time of selection (td) • ancestral population size (N2) • and current population size (N0) on the expected level of polymorphism

  15. Simulation 3

  16. Simulation 4 • Individual Polymorphisms in 8 different simulations • Polymorphism decreases as you get further away from the target site • Target site is at 0.5

  17. Simulation 4

  18. Experimental Methods Statistical Tests for Selection • Tajima’s (D) • Fay and Wu’s (H) • Hudson – Kreitman – Aguade (HKA) These statistical tests were used to analyze the simulations to detect a signature of selection. These tests supported the theoretical model in that it followed the patterns of polymorphism and selection.

  19. Discussion and Implications • Models were developed to measure the level of polymorphism and subsequently detect genes that were selected for through domestication events. • Initial frequencies of alleles greatly affects the likelihood that evidence for selection can be detected from patterns of polymorphism. • Difficult to detect many genes involved in domestication • It is likely that these patterns will be used to detect domesticated genes in future studies, but a more robust model will be needed in cases when the initial p is high. • Implication previously discussed • Crop yields • Diseases

More Related