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Lab 9: Dna eLECTROPHORESIS

Lab 9: Dna eLECTROPHORESIS. TCTC Bio 101 Lab Rinehart . Structure of DNA. Nucleic acid with Nitrogenous base 5-C sugar Phosphates Nature of the molecule confers polarity!!! If it’s polar – then it acts as a magnet. Structure of DNA. Nucleic acid with Nitrogenous base 5-C sugar

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Lab 9: Dna eLECTROPHORESIS

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  1. Lab 9: DnaeLECTROPHORESIS TCTC Bio 101 Lab Rinehart

  2. Structure of DNA • Nucleic acid with • Nitrogenous base • 5-C sugar • Phosphates • Nature of the molecule confers polarity!!! • If it’s polar – then it acts as a magnet

  3. Structure of DNA • Nucleic acid with • Nitrogenous base • 5-C sugar • Phosphates • Nature of the molecule confers polarity!!! • If it’s polar – then it acts as a magnet

  4. Structure of DNA

  5. Creating the Fragmemnts • Scientists discovered bacterial enzymes that digest foreign DNA to protect themselves • We use these “restriction enzymes,” that cleave DNA strands at known base pair patterns or repeats, to “cut” our strands into fragments.

  6. The Process

  7. Viewing a Gel!! • Using your ladder to create a standard curve • Estimation of base pairs in fragments based on standard curves

  8. “Who The Baby Daddy?”

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