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Instrumentation and control

Instrumentation and control. Parameters: to be monitored and controlled Temperature Pressure Agitator shaft power Flowrate Liquid level Viscosity Turbidity pH Redox potential Ion concentration DO Read pages 308-310 in the text book. Summary of Bioreactor Design and Operation.

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Instrumentation and control

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  1. Instrumentation and control • Parameters: to be monitored and controlled • Temperature • Pressure • Agitator shaft power • Flowrate • Liquid level • Viscosity • Turbidity • pH • Redox potential • Ion concentration • DO Read pages 308-310 in the text book.

  2. Summary of Bioreactor Design and Operation • Modified batch and continuous reactors - Chemostat with cell recycle Keep high cell mass concentration in the reactor. Production of biomass and low-value product - Fed-batch Maintain low substrate concentration Secondary metabolites, prevent catabolite repression - Multi-stage chemostat reactor Separate cell growth and product formation Secondary metabolites, genetically modified cell culture

  3. Summary of Bioreactor Design and Operation • Modified batch and continuous reactors - Chemostat with cell recycle (qp=0, kd ≈0, X0=0,Monod equation is applied):

  4. Summary of Bioreactor Design and Operation • Modified batch and continuous reactors - Fed-batch (qp=0, kd ≈0, Monod equation is applied):

  5. Summary of Bioreactor Design and Operation • Modified batch and continuous reactors - Multi-stage chemostat reactor

  6. Summary of Bioreactor Design and Operation • Immobilized cell system Advantages and disadvantages • Operation consideration agitation and aeration, determination of volumetric mass transfer coefficient kLa, heat removal, foam, etc.

  7. Summary of Bioreactor Design and Operation • Scale up/down: geometric and dynamic similarity: In scale-up/down of a stirred-tank reactor, the design calculations are as follows: • Determine the scale-up/down factor Dp/Dm • Calculate the dimensions of the prototype (height H and diameter Dt of tanks, impeller diameter Di) by multiplying that of the model with the scale-up/down factor. - Select criterion related to dynamic properties and keep it constant in both the model and the prototype. - Determine the parameters such as impeller speed for the scale-up/down reactor.

  8. Summary of Bioreactor Design and Operation • Sterilization liquid: thermal inactivating 1- P0(t)= 1-[1-e-kdt]N0 kd = αe-E0d/RT From the above equation: • Known N0, T, t, determine Kd, the probability of an unsuccessful sterilization is determined. • Given N0, T, acceptable probability of failure e.g. 10-3, required time can be determined • Higher Kd tends to achieve low probability of sterilization failure. Normally at 121oC. Kd of vegetative cells > 1010 min-1, spores 0.5-5 min-1. The major concern is spores.

  9. Summary of Bioreactor Design and Operation • Sterilization Degradation of important compounds in the medium by thermal inactivating ln C/C0=-kdt where C and C0 are concentrations of the component at time t and t=0, respectively. kd is the degradation rate constant. kd = αe-E0d/RT To determine the components remaining active: the temperature T → determine kd → with known t, determine C.

  10. Summary of Bioreactor Design and Operation • Bioreactor Instrumentation and control

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