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Kari Haajanen tuntiopettaja, laboratoriopäällikkö Turun AMK

GMO-ruokaa vai ei?. Kari Haajanen tuntiopettaja, laboratoriopäällikkö Turun AMK. Realismi Idealismi T ietoisuus. GMO ja elintarvikkeet. HS 24.4.2014. Turun AMK 1992–2014. GMO- laboratoriotestin kulku.

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Kari Haajanen tuntiopettaja, laboratoriopäällikkö Turun AMK

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  1. GMO-ruokaa vai ei? Kari Haajanentuntiopettaja, laboratoriopäällikköTurun AMK

  2. Realismi Idealismi Tietoisuus GMO jaelintarvikkeet

  3. HS 24.4.2014

  4. Turun AMK 1992–2014

  5. GMO-laboratoriotestinkulku

  6. Test is based on the detection and amplification of gene regions specific for GMOs (35S promoter and NOS terminator), and not present in native plants. • 90 % of the GMOs that have been commercialised up to date present this specific regions. • 35S promoter of the cauliflower mosaic virus. The promoter serves as adocking site for RNA polymerase and a signal for where it should start transcribing a gene. • Terminator of the nopaline synthase (NOS) gene of Agrobacterium tumefaciens (Gram-negativebacteria). The terminator is the signal to stop transcription.

  7. DNA:neristys

  8. DNA:nmittaus: • Suhdeluku A260/A280 1,8 = puhdas DNA • Mittakaavan pienuus aiheuttaa haasteita

  9. PCR-vaiheet

  10. PCR-pipetointi http://www.dnalc.org/resources/animations/pcr.html

  11. PCR:n idea

  12. PCR:nkopiointikykyon “rajaton”

  13. Agaroosigeelielektroforeesi http://www.dnalc.org/resources/animations/gelelectrophoresis.html

  14. AGE:nperiaate

  15. AGE-tulos

  16. GMO-testintulos

  17. Luettavaa + materiaalia aiheen tiimoilta: • First fruit (flavr savr) • Europeans and biotechnology in 2010 • Biogeeni • Geenitekniikka

  18. KiitosKysymyksiä?

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