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Fluorescence to the Rescue: Understanding Flow Cytometry for Cell Separation

Explore the basic excitation and emission characteristics of common dyes in flow cytometry for cell separation. Learn about emission spectra, data analysis, and the architecture of a FACS Calibur instrument's cell sorting module. Discover the lining up of cells in scatter light dot plots of lysed blood for efficient cell separation.

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Fluorescence to the Rescue: Understanding Flow Cytometry for Cell Separation

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Presentation Transcript

  1. FLOW CYTOMETRY FOR CELL SEPARATION

  2. Cells Scatter Light

  3. Dot Plot Of Light Scatter Of Lysed Blood

  4. Fluorescence To The Rescue!

  5. Basic Excitation/Emission Characteristics Of Common Dyes

  6. Emission Spectra

  7. Analyzed Data

  8. Architecture Of A FacsCalibur Instrument

  9. Cell Sorting Module (FacsCalibur)

  10. Lining Up Of Cells

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