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Validation of Alere TM q HIV-1/2 Detect for detection of acute HIV infection

Validation of Alere TM q HIV-1/2 Detect for detection of acute HIV infection at Anonymous Clinic, The Thai Red Cross AIDS Research Centre.

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Validation of Alere TM q HIV-1/2 Detect for detection of acute HIV infection

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  1. Validation of Alere TM q HIV-1/2 Detect for detection of acute HIV infection at Anonymous Clinic, The Thai Red Cross AIDS Research Centre IrinSrila-or1, TippawanP.1, SupanitP.1, SupphadithB.1, PanatsiriR.1, JureepornJ.1, SuphachokeA.1, SuneeS.2, PattareeyaP.1, ReshmieR.1, NittayaP.1,3, PraphanP.1,2 1. The Thai Red Cross AIDS Research Centre, Bangkok, Thailand. 2. Faculty of Medicine, Chulalongkorn University, Bangkok, Thailand. 3. SEARCH, Bangkok, Thailand

  2. Introduction • Diagnosis of acute HIV infection (AHI) is important for prevention and care. • Nucleic acid amplification test (NAAT) is the most sensitive test to diagnose AHI but is costly and labor intensive. • AlereTMq is a point-of-care qualitative NAAT that can detect both HIV type 1 and 2 with a detection limit of 2,500 copies/mL • Aim of the study is to evaluate the performance of Alereq in Thai AHI detection.

  3. Methods & Results Table : Alereq HIV-RNA Detectable stratified by group Alereq was run against a panel of one hundred plasma samples : • Confirmed HIV negative (10) • 4th Gen. EIA non-reactive but NAAT (APTIMA) reactive (30) • 4th Gen. reactive but 3rd Gen. non-reactive (30) • 4th Gen. and 3rd Gen. reactive but 2nd Gen. non-reactive (30) • * 8/9 Alere q negative samples had HIV-RNA below 2,500 Copies/mL, the detection limit of Alere q, the remaining one had HIV-RNA 40,311 copies/mL. • ** HIV-RNA of the only Alereq negative sample in group D was only 1,204 copies/mL. • *** Median HIV-RNA of AHI with reactive Alere q was 630,276 copies/mL which was significantly higher than those with non-reactive Alere q (282 copies/mL), p<0.001.

  4. Conclusions • Alere q is more sensitive than 4th Gen. machine base EIA but less sensitive than APTIMA. • Alere q is ideal for HIV testing in people taking PrEP especially at community roll out. • In addition to its indication for use in mother-to-child transmission, Alere q could be considered in the detection of AHI in a setting where APTIMA or HIV-RNA is not available although its sensitivity is not yet perfect.

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