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Enhancing the Efficiency of a Polymerase Chain Reaction Using Au Nanoparticles

Enhancing the Efficiency of a Polymerase Chain Reaction Using Au Nanoparticles The Goal of our studies

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Enhancing the Efficiency of a Polymerase Chain Reaction Using Au Nanoparticles

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  1. Enhancing the Efficiency of a Polymerase Chain Reaction Using Au Nanoparticles

  2. The Goal of our studies • Nanoparticles show excellent heat transfer properties, and rapidly dissipate energy to their surroundings. These properties were used to enhance the efficiency of real-time polymerase chain reaction (real-time PCR), and PCR reaction. • Gold colloid with a diameter of 13nm was added into the polymerase chain reaction.

  3. Mechanisms of heat flow in suspensions of nano-sized

  4. DNA fragment view EGFP-N GFP Sequences EGFP-C About 630bp EGFP-N-S GFP-IS EGFP-N-AS About 150bp EGFP-N-S (5-TGCAGTGCTT -CAGCCGCTAC-3) EGFP-N-AS (5’-CAGCTCGATGCGGTTCACCA-3’),

  5. Au nanoparticles were not changed after real-time PCR. • Detection of PCR products before and after real-time PCR by UV-vis spectrometer. Both have absorbance in 520 nm, same as the spectrum in Figure 1B, representing the 13nm of gold colloid, a peak was detected. • (B) TEM image shows the Au nanoparticles were no change (13 nm) after real-time PCR.

  6. The effect of Au nanoparticles on PCR efficiency The positive control group was compared with the Case D group, in which 7.52×10-7 mM of gold particles were added. The reference PCR time used was denaturing/95C, 15s/, annealing/58C, 15s/, extending /72C, 30s. (A) The positive control group; (B) Case D group; (C) The reaction time was reduced to 2/3 of the reference PCR time. (D) The same as Fig. 4C, in case D the reaction time was reduced to 2/3 of the PCR reaction time. (E) The reaction time was reduced to 1/3 of the reference PCR time. (F) The PCR reaction time was reduced to 1/3 of Fig. 4D.

  7. The effect of Au nanoparticles on the efficiency of PCR reaction of BNIP3 cDNA.

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