1 / 8

SPECTROPHOTOMETRY

SPECTROPHOTOMETRY. Principle :. there is interaction between the light and sample particle , spectrophotometer is employed to measure the amount of light that a sample absorbs. spectrophotometer consists of two instruments: 1- spectrometer

bernadettea
Download Presentation

SPECTROPHOTOMETRY

An Image/Link below is provided (as is) to download presentation Download Policy: Content on the Website is provided to you AS IS for your information and personal use and may not be sold / licensed / shared on other websites without getting consent from its author. Content is provided to you AS IS for your information and personal use only. Download presentation by click this link. While downloading, if for some reason you are not able to download a presentation, the publisher may have deleted the file from their server. During download, if you can't get a presentation, the file might be deleted by the publisher.

E N D

Presentation Transcript

  1. SPECTROPHOTOMETRY

  2. Principle: there is interaction between the light and sample particle , spectrophotometer is employed to measure the amount of light that a sample absorbs .

  3. spectrophotometer consists of two instruments: 1- spectrometer for producing light of any selected color (wavelength 2- photometer for measuring the intensity of light. The instruments are arranged so that liquid in a cuvette can be placed between the spectrometer beam and the photometer

  4. The most common spectrophotometers are used in the UV and visible regions of the spectrum .

  5. Light source : Two kinds of lamps 1-Deuterium lamp in UV- spectrophotometer spectrophotometer- 2-tungsten lamp in visible

  6. Sample cell : A container that contains a sample is usually called "cell“ two types are available: 1-glassused in visible spectrophotometer 2-quartzused in UV spectrophotometer .

  7. Application : 1- qualitative : The wavelength of maximum conc. give information about structure in λ max 2- quantitative : Determination of conc. By beer’s law A = abc

  8. Sample and standard solution : To measure the concentration of a substance, a sample of (unknown concentration) is compared with known concentration (standard solution) to determine the concentration . The blank contain all the substance exceptthe analyte

More Related