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Rapid Hygiene Monitoring

Learn about the principles of bioluminescence and ATP in hygiene monitoring, and how ATP bioluminescence can provide rapid and accurate results for assessing cleanliness. Discover the benefits of using ATP bioluminescence for monitoring hygiene and its application in various industries.

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Rapid Hygiene Monitoring

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  1. Rapid Hygiene Monitoring

  2. Taking control of Hygiene Immediately & Effectively!

  3. PRINCIPLE WHAT IS BIOLUMINESCENCE? Non-thermal light produced by living organisms as a result of metabolic processes at cellular level. (Greek) Bios = “Living” (Latin) Lumen = “Light” (The company name ‘Bio-Glo’ is derived from this concept)

  4. PRINCIPLE WHAT IS ATP? Abbreviation for Adenosine Tri-Phosphate A chemical substance that acts as an energy source for all living organisms on the planet. The presence of ATP can be considered proof of the presence of a living organism, or of a substance produced by a living organism.

  5. Different Types of ATP Somatic ATP Free ATP Bacterial ATP TOTAL ATP

  6. Different Types of ATP Somatic ATP Free ATP Bacterial ATP BACK GROUND ATP

  7. MetabolicProcess ATP

  8. IN VIVO - IN VITRO

  9. ATP vs.LIGHT(RLU) Calibration curve for ATP 106 Direct correlation between ATP and LIGHT 105 104 Bioluminescence (RLU) 103 102 101 10-17 10-16 10-15 10-14 10-13 10-12 ATP (mol/assay)

  10. Conventional Method > 48 HOURS !

  11. Very rapid Hygiene Monitoring by ATP Bioluminescence …days Traditional Plate Method Check only ３０ sec. ATP Method Check

  12. Because it takes… >100 000 cells to make 1 visible colony (CFU) <1 000cells to obtain a reading (RLU)

  13. TVC vs. ATP Traditional Plate Method Microorganism ATP Bioluminescence Method Food residue Judged Dirty Cuttingboard Judged Dirty Neither sufficient washing nor sterilization. Food residue Judged Clean Cutting board Judged Dirty Insufficient washing but enough sterilization. As time passes, microorganisms increase up to a dangerous level. Judged Clean Judged Clean Cuttingboard Completely clean!

  14. TVC Method Time/Product release Complex methods Special lab facilities Visible colony

  15. BENEFITS – Production • Results in real time allows for immediate remediation. • Tests & sanitizing protocols can be repeated until preferred levels are obtained. • Sensitivity – better possibility of picking up low levels of contamination. • Tests are easy to perform • Suitable for training of cleaning staff. • Instruments may be interfaced to a PC.

  16. Selection of Instruments Single-Shot DeviceSuitable for surface monitoring.

  17. Instrument and swabs Lumitester PD-10N The integrated swab

  18. ATP Bioluminescence 48 Hours 2 minutes 2 minutes

  19. Examples in Nature

  20. MAKELIGHTWORK!

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