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At18S-Adapter.R1

Analysis of the ccmC t-element. A. ccmC. 3’. 5’. (-484/-482). (+37). ccmC pre-mRNA. ccmC mRNA. (-46/-45). ccmC t-element. At18S-Adapter.H1. Atccb3-Mega.3’.fern. 18S rRNA. 18S rRNA. ccmC t-element. Atccb3-Endo-3’.R. Atccb3-10. At18S-Adapter.R2. At18S-Adapter.R1. B. C.

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At18S-Adapter.R1

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  1. Analysis of the ccmC t-element A ccmC 3’ 5’ (-484/-482) (+37) ccmC pre-mRNA ccmC mRNA (-46/-45) ccmC t-element At18S-Adapter.H1 Atccb3-Mega.3’.fern 18S rRNA 18S rRNA ccmC t-element Atccb3-Endo-3’.R Atccb3-10 At18S-Adapter.R2 At18S-Adapter.R1

  2. B C mt RNA marker 2,000 bp 1,500 bp 1,000 bp 750 bp 500/501/489 bp I 404 bp 331 bp 250/242 bp D

  3. E F mt RNA marker 2,000 bp 1,500 bp 1,000 bp 750 bp 500/501/489 bp II 404 bp 331 bp 250/242 bp G

  4. Supplementary Figure 30. Analysis of the ccmC t-element. A modified CR-RT-PCR using endogenous 18S rRNA as adapter molecule was carried out to determine the 5’ end (B-D) and the 3’ end (E-G) of the t-element downstream of the ccmC transcript (A). The site of the endonucleolytic cleavage of the pre-mRNA is marked by a pair of scissors. Primers indicated in tables (C) and (F), respectively, were used and the PCR products were separated by agarose gel electrophoresis (B and E). The products (marked by an arrow) were excised and analyzed by sequencing. The ends identified within the PCR products are given in tables (D) and (G), respectively.

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