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RNAi - Technologies, Markets and Companies

Bharat Book Presents"RNAi - Technologies, Markets and Companies" compares RNAi with other antisense approaches using oligonucleotides, aptamers, ribozymes, peptide nucleic acid and locked nucleic acid.

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RNAi - Technologies, Markets and Companies

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  1. RNAi - Technologies, Markets and Companies Bharat Book Bureau

  2. Summary RNA interference (RNAi) or gene silencing involves the use of double stranded RNA (dsRNA). Once inside the cell, this material is processed into short 21-23 nucleotide RNAs termed siRNAs that are used in a sequence-specific manner to recognize and destroy complementary RNA. The report compares RNAi with other antisense approaches using oligonucleotides, aptamers, ribozymes, peptide nucleic acid and locked nucleic acid.Various RNAi technologies are described, along with design and methods of manufacture of siRNA reagents. These include chemical synthesis by in vitro transcription and use of plasmid or viral vectors. Other approaches to RNAi include DNA-directed RNAi (ddRNAi) that is used to produce dsRNA inside the cell, which is cleaved into siRNA by the action of Dicer, a specific type of RNAse III. MicroRNAs are derived by processing of short hairpins that can inhibit the mRNAs. Expressed interfering RNA (eiRNA) is used to express dsRNAintracellularly from DNA plasmids.

  3. Continue… Delivery of therapeutics to the target tissues is an important consideration. siRNAs can be delivered to cells in culture by electroporation or by transfection using plasmid or viral vectors. In vivo delivery of siRNAs can be carried out by injection into tissues or blood vessels or use of synthetic and viral vectors.Because of its ability to silence aanygene once the sequence is known, RNAi has been adopted as the research tool to discriminate gene function. After the genome of an organism is sequenced, RNAi can be designed to target every gene in the genome and target for specific phenotypes. Several methods of gene expression analysis are available and there is still need for sensitive methods of detection of gene expression as a baseline and measurement after gene silencing. RNAi microarray has been devised and can be tailored to meet the needs for high throughput screens for identifying appropriate RNAi probes. 

  4. Table of Contents 0. Executive Summary . 151. Technologies for suppressing gene function........ 17Introduction ........17DNA transcription..........17RNA ......17Non-coding RNA ......17RNA research and potential applications.......18Role of RNA in regulation of the dihydrofolatereductase gene .........19Gene regulation.19Post-transcriptional regulation of gene expression.........20Alternative RNA splicing.21Technologies for gene suppression.......21Antisense oligonucleotides ........22Phosphorodiamidatemorpholinooligomers........22Transcription factor decoys .......22Aptamers..........23

  5. Related Reports • Antiviral Therapeutics - Technologies, Markets and Companies • Competitor Analysis: Anthrax Vaccines & Therapeutics • Pain Therapeutics - Drugs, Markets and Companies • Nitric Oxide - Therapeutics, Markets and Companies • Nanotherapeutics, Inc. - Product Pipeline Review - 2013

  6. About us : Bharat Book Bureau is the leading market research information aggregator that provides market research reports, industry analysis, company profiles, business reports, country reports, newsletters and online databases. Our clients include Corporate, Consulting firms, and Academic Institutions and Government departments across the globe. Bharat Book Bureau provides over a million reports from more than 400 publishers around the globe. The market research reports we provide, help global companies examine the different markets before setting up a business or expanding into different countries across the world. They give a complete perspective on the current market scenario, trends, segments and future outlook.

  7. For More Information You Can Visit- http://www.bharatbook.com/market-research-reports/healthcare-market-research-report/rnai-technologies-markets-and-companies.htmlOr visit us at: Bharat Book Bureau Phone : +91 22 27810772, 27810773Call UK: +44 7405 208461 Toll Free No for USA/Canada : 1-866-279-8368 E-mail : info@bharatbook.com Website : www.bharatbook.com Follow us on : Request Sample Pages

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