PROF. SYED QAISER ABBAS EX-Chairman Department of Botany GC University, Faisalabad

1. IDENTIFICATION OF SOME COMMON GENERA OF COELOMYCETES PROF. SYED QAISER ABBASEX-Chairman Department of BotanyGC University, Faisalabad

2. Identification of Some Common Genera of Coelomycetes • Basis of identification • CONIDIOMATAL STRUCTURES • CONIDIA & CONIDIOGENESIS

3. CONIDIOMATAL STRUCTURES (Six variants morphological types) • Phoma-type(Conidiomata Pycnidial, dark colored, leathery to carbonaceous, stromatic or non-stromatic pycnidia generally with a circular ostiole) Zythia (Nectria)-type(Conidiomata Pycnidial as in Phoma-type, but light colored, and sometimes waxy) • Leptostroma-type(Conidiomata shield-shaped) • Discella-type(Conidiomata more or less cup shaped) • Pycnothyrial Type(Conidiomata pycnothyrium) • EustomataType (Conidiomata Eustomatic, Multilocular of complex structure of different shapes)

4. Types of Conidiomata • Hyphal: Conidia are formed on hyphae. • Sporodochial: Conidiophore aggregate in a manner that they formed a cushion-like structure. • Synnemata: The conidiophores aggregate in a manner that they are forming a bundle of sticks-like structure. • Acervulus: The conidiophores are formed on saucer-like structure. • Pycnidium: The conidia and conidiophore are formed in closed unilocular body. • Eustromata: The conidiomata are multilocular and conidia are formed inside the body.

5. Conidiogenesis: A process of conidia formation. • Three types(Hennbert and Sutton, 1994) • Blastic:(Conidia mature first then septa formation occurs) • Thallic: (Septa formed first then conidia mature) • Thalloblastic: (Intermediary between Blastic and Thallic conidiogenesis, Conidiogenous cells slightly increase in shape and size then septa formation occur, afterward maturation of conidia occurs)

6. Conidiogenesis:

7. Conidia Formation in relation to wall • Hologenous: Conidial wall formed by two layer of conidiogenous cells • Enterogenous: Conidial wall formed by only inner wall of conidiogenous cells which forms the outer wall of the conidia and inner wall of conidia formed, from the conidial cell contents • Endogenous: Conidial wall of conidia formed by cytoplasmic content of cell or structure where they are formed.

8. Conidia Formation in relation to wall

9. Process of Conidiogenesis(Involves Five Steps) 1:- Initiations: first step in conidiogenesis, enzymes involved in wall building become very active and according to conidial development pattern, apical wall building material or lateral wall building materials becoming available and increase in size and in shape become evident. 2:- Maturation: Conidia acquire their full shape and size. 3:- Delimitation: Conidia are separated by septa formation. However, this depends upon thallic, blastic or thalloblastic type of conidiogenesis when it has to be formed.

10. Process of Conidiogenesis 4:- Seccession: Separation of conidia from conidiogenous cell. • Schizolytic: Separation of conidia occurs by two pile septum in a way that conidia and conidiogenous cells both have half part of septum • Rhexolytic: conidia separate from conidiogenous cells in a way that both part remain attached to conidia hence conidiogenous cell splits just below the septum, which separate conidia and conidiogenous cell.

11. Process of Conidiogenesis 5:- Proliferation, or successive development of conidial loci • Generally only a single conidium is produced at a locus, however, in many cases a number of conidia are produced simultaneously or successively at newly developing loci .The process is called proliferation and of three types. 1 Progressive proliferation: Length of conidiogenous cells increases. 2 Stationaryproliferation: Length of conidiogenous cells does not increases. 3 Retrogressive proliferation Length of conidiogenous cells decreases.

12. Process of Conidiogenesis

13. Identification of Some Common Genera of Coelomycetes 1 Coniothyrium Cde. • Conidiomata …………Pycnidial • Conidiophore …………… Absent. • Conidiogenous cell………Blastic,hologenous and progressive. • Conidia………………… Unseptate,brown and not enclosed in a mucilaginous sheath.

14. Coniothyrium Cde.

15. Sphaeropsis Sacc. Conidiomata …………Pycnidial • Conidiophore ………… Absent • Conidiogenous cell………Blastic,hologenous, non-proliferating but some time with one proliferation • Conidia………………… Unseptate, brown to black not enclosed in a mucilaginous sheath.

16. Sphaeropsis Sacc.

17. Microsphaeropsis Hohn • Conidiomata …………Pycnidial • Conidiophore …………… Absent • Conidiogenous cell……… Blastic, enterogenous and stationary • Conidia……………………Unseptate, brown to black not enclosed in a mucilaginous sheath.

18. Microsphaeropsis Hohn

19. Avetteae Petrak & Syd. • Conidiomata …………Pycnidial to eustromatic • Conidiophore …………… Absent or rearly of 1-3 celled. • Conidiogenous cell………Blastic, hologenous and progressive. • Conidia…………………Unseptate, brown to black and enclosed in a mucilaginous sheath.

20. Avetteae Petrak & Syd.

21. Phoma Sacc. • Conidiomata …………Pycnidial • Conidiophore ……………Absent are rarely of one celled. • Conidiogenous cell………Blastic, enterogenous and stationary • Conidia…………………Unicellular hyaline and non enclosed in mucilagenous sheath

22. Phoma Sacc

23. Phyllosticta Desm. • Conidiomata …………Pycnidial • Conidiophore ……………Absent are rarely of one celled. • Conidiogenous cell……Non proliferating and, some time proliferate enterogenous and stationarly • Conidia…………………Unseptate, hyaline enclosed in mucilagenous sheath

24. Phyllosticta Desm.

25. Ascochyta Lib. • Conidiomata …………Pycnidial • Conidiophore ……………Absent • Conidiogenous cell…… Enterogenous and stationary • Conidia……………… Uniseptate and hyaline

26. Ascochyta Lib.

27. Diplodia Fr. • Conidiomata ……… Pycnidial • Conidiophore ………… Branched and septate about and unbranched cylindrical at the base • Conidiogenous cell……Blastic, hologenous and non proliferating • Conidia……………………1 septate, septa transverse and brown to black

28. Diplodia Fr.

29. Hendersonia Berk. • Conidiomata …………Pycnidial • Conidiophore …………… Absent are rarely of one celled. • Conidiogenous cell……… Blastic, hologenous and prograssive. • Conidia………………… Multiseptate, septa transverse and brown with out appendages

30. Urohendersonia Speg. (Fig. 15) • Conidiomata …………Pycnidial • Conidiophore ……………Absent are rarely of one celled. • Conidiogenous cell…… .Blastic, hologenous and prograssive. • Conidia…………………Multiseptate, septa transvers and brown with one apical appendage

31. Urohendersonia Speg.

32. Seimatosporiopsis Sutton,Ghaffar & Abbas. • Conidiomata ……… Pycnidial to eustromatic • Conidiophore ………… Absent • Conidiogenous cell…… Blastic, hologenous and prograssive. • Conidia………………… Multiseptate with 3-4 transvers septa and brown with one apical and basal appendage

33. Seimatosporiopsis Sutton,Ghaffar & Abbas.

34. Camarosporium Schulz. • Conidiomata …………Pycnidial • Conidiophore ……………Absent • Conidiogenous cell…… Blastic, hologenous and prograssive. • Conidia………………… Multiseptate, muriform, brown

35. Camarosporium Schulz.

36. Thanks for Sparing Your Precious

37. Best wishes for a Rewarding Career in Mycology & Plant Pathology