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Analysis of Env binding of Fluor-conjugated antibodies (A. Kliche ) . Ab. Binding to ConS gp140 #86. ConS gp140 #86 binding to A32. RU. RU. 300. 400. B12 - A32. 350. 250. A32 . A32. 300. B12-ALEXA488- A32. 200. A32. 250. 150. 200. 150. 100. 100. Response. Response.
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Analysis of Env binding of Fluor-conjugated antibodies (A. Kliche)
Ab Binding to ConS gp140 #86 ConS gp140 #86 binding to A32 RU RU 300 400 B12 - A32 350 250 A32 A32 300 B12-ALEXA488- A32 200 A32 250 150 200 150 100 100 Response Response Req ConS 140 #86 - A32 332 ConS 140 #86 - A32 331 ReqAb binding ratio ConS 140 #86 + B12 - A32 274 0.825 ConS 140 #86 +B12-ALEXA488- A32 250 0.755 50 50 0 0 ConS 140 -50 -50 -50 -50 0 0 50 50 100 100 150 150 200 200 250 250 Time Time s s Control IgG A32 Control IgG
Ab ConS gp140 #86 Binding to ConS gp140 #86 RU RU HGN194-ALEXA488 300 350 300 250 HGN194 A32 A32 A32 250 200 200 150 150 100 ReqAb binding ratio ConS 140 #86 + HGN194 - A32 265 0.81 ConS140+HGN194-ALEXA488 - A32 278 0.894 Req ConS 140 #86 - A32 327 ConS 140 #86 - A32 311 100 Response Response 50 50 ConS 140 0 0 -50 -50 -50 -50 0 0 50 50 100 100 150 150 200 200 250 250 Time Time s s A32
300 350 ConS 140 CFI Capture ConS 140 CFI Capture 250 ConS 140 CFI Capture 350 250 HJ16 200 HJ16 250 300 HJ16 200 300 250 200 200 150 Binding Response (RU) 250 HJ16Alexa Fluor 488 150 200 150 HJ16Alexa Fluor 488 150 HJ16Alexa Fluor 488 200 100 150 100 100 100 150 100 50 50 50 100 50 50 0 50 0 0 0 0 -50 -50 0 50 100 150 200 250 300 350 0 -50 -50 -50 -50 0 50 100 150 200 250 -50 -100 0 100 200 300 400 -50 -50 0 50 100 150 200 250 -50 0 50 100 150 200 250 300 Binding Response (RU) -50 0 50 100 150 200 250 Time (s) Time (s) Time (s)
