1 / 2

Localisation of a Calmodulin-Binding Site

Localisation of a Calmodulin-Binding Site. 1. 581. GST-fusion protein pull down of CaM1234. Lbs-1. W226. 581. Lbs-1  1-225. pGST GST-Cyt1 GST-Cyt2. 309. 159. 1. 50 M f ree Ca 2+. Cyt1 Cyt2. CaM1234. 1 mM EGTA. CaM1234.

lexi
Download Presentation

Localisation of a Calmodulin-Binding Site

An Image/Link below is provided (as is) to download presentation Download Policy: Content on the Website is provided to you AS IS for your information and personal use and may not be sold / licensed / shared on other websites without getting consent from its author. Content is provided to you AS IS for your information and personal use only. Download presentation by click this link. While downloading, if for some reason you are not able to download a presentation, the publisher may have deleted the file from their server. During download, if you can't get a presentation, the file might be deleted by the publisher.

E N D

Presentation Transcript

  1. Localisation of a Calmodulin-Binding Site 1 581 GST-fusion protein pull down of CaM1234 Lbs-1 W226 581 Lbs-1 1-225 pGST GST-Cyt1 GST-Cyt2 309 159 1 50 M free Ca2+ Cyt1 Cyt2 CaM1234 1 mM EGTA CaM1234

  2. Detailed localisation using peptides 159 1 1-5-10 1-5-10 53% IQ 1-5-8-14 76% IQ 70% IQ (site1) A C D E B A B C D E F CaM F A B C D E F CaM Ca2+ EGTA 1- B/Bo

More Related