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primary carcinoma

Monoclonal Antibody Microarray. mRNA profile – pathway clustering. primary carcinoma. metastasis. LMP. Monoclonal Antibody Microarray. Protein profile – pathway clustering. In situ Protein Array Technology (ISPAT):. T7. GOI. TUS. HIS. TER.

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primary carcinoma

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  1. Monoclonal Antibody Microarray • mRNA profile – pathway clustering primary carcinoma metastasis LMP

  2. Monoclonal Antibody Microarray • Protein profile – pathway clustering

  3. In situ Protein Array Technology (ISPAT): T7 GOI TUS HIS TER • Partnership with PEL (Drs. Deb Chattergee + James Hartley) • Protein microarrays ‘On demand” • ‘Print’ DNA expression construct – in vitro transcription / translation GOI GST (Ramachandran, LaBaer 2004) Protein Kobs (M) T1/2 (min) Tus wt 7-8 X 10 -13 160 Tus (E47Q) 350 GFP-Tus (E47Q) 3-5 X 10-12 800 2 X 10 -13

  4. In situ Protein Array Technology (ISPAT): Cytochrome C Oxidade sub IV Importin alpha 3 Synovial Sarcoma ADP- ribosylation factor 6 ChK2 Kinase homolog Integrin beta- binding protein GFP GFP F-box & WD 40 No TER T7 GOI TUS HIS TER • TER specific binding of proteins • Full-length translation • No ‘bleed-through’ - TER No Ter + TER Mutant + TER Wild-type anti-His anti-GFP

  5. Laboratory of Molecular Technology SAIC-Frederick Larry Arthur Bruce Crise Denise Whitby Deb Chatterjee James Hartley NCI Robert Callahan Karlyne Reily Margaret Tucker Alicia Goldstein Maria Landi

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