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Supplementary Figure Captions. Fig. S1 Overview of small RNA sequencing data
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Supplementary Figure Captions Fig. S1 Overview of small RNA sequencing data a Data cleaning of small RNA sequencing; b Mapping statics of small RNA; c Distribution of small RNA on each chromosome; d Summary of common and specific sequences between sr-Tubers4C and sr-Tubers20C; e small RNA annotation Fig. S2 Overview of known miRNAs expressed in potato tubers a Length distribution of mature miRNA sequences; b miRNA nucleotide bias at each position; c Distribution of pre-miRNAs minimal folding free energy; d Distribution of pre-miRNAs length Fig. S3 Overview of novel miRNAs identified in potato tubers a Length distribution of mature miRNA sequences; b miRNA nucleotide bias at each position; c Number of miRNAs for each novel miRNA; d Distribution of pre-miRNAs minimal folding free energy; e Distribution of pre-miRNAs length Fig. S4 Overview of degradome sequencing data a Data cleaning of degradome sequencing; b Length distribution of degradome sequences; c Mapping statics of degradome sequences; d Distribution of degradome sequences on each chromosome; e Summary of common and specific sequences between deg-Tubers4C and deg-Tubers20C; f Degradome sequences annotation Fig. S5 Expression patterns of the cold-responsive miRNAs/miRNA*s and their targets Potato tubers were stored at 4 °C and 20 °C, and sampled at 0 d (T0), 1 d, 2 d, 5 d, and 15 d. The expression patterns of miRNAs/miRNA*s were determined by stem-loop RT-qPCR and the expression patterns of targets were determined by RT-qPCR. The results were illustraited by MeV 4.8 software. The expression level of each miRNA/target at T0 was set as 0 for 10908-06 and ED25, respectively. The name or the accession number and the abbreviated annotation of the miRNAs/targets were shown on the right. The color scale for logarithm signal values is shown on the top
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stu-miR166 PGSC0003DMT400006735, HD-ZIP Ⅲ PGSC0003DMT400068037, HD-ZIP Ⅲ PGSC0003DMT400074932, HD-ZIP Ⅲ stu-miR172 PGSC0003DMT400031401, RAP2 PGSC0003DMT400065313, AP2 PGSC0003DMT400018466, DOLK PGSC0003DMT400042918, DCL PGSC0003DMT400054945, BEL29 stu-miR396 PGSC0003DMT400034693, GAD PGSC0003DMT400061170, SnRK2.4 stu-miR396a-3p PGSC0003DMT400001086, IRX7 PGSC0003DMT400049314, PDR stu-miR482f-5p PGSC0003DMT400069706, SUO 10908-06 ED25 20°C 4°C 20°C 4°C T0 1d 2d 5d 15d 1d 2d 5d 15d T0 1d 2d 5d 15d 1d 2d 5d 15d Fig. S5