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Protozoa

Protozoa. Lab. : 14 Ciliates Balantidium coli Kinetoplastida 1. Leishmania donovani. Ciliates Balantidium coli. Balantedium coli A. trophozoite : shape ovoid; cilia cover the body; cytostom present; nuclei 1 small (micronucleus), 1 large ( macronuclues ).

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Protozoa

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  1. Protozoa

  2. Lab. : 14 Ciliates Balantidium coliKinetoplastida1. Leishmaniadonovani

  3. Ciliates Balantidium coli

  4. Balantedium coli A. trophozoite:shape ovoid; cilia cover the body;cytostom present; nuclei 1 small (micronucleus), 1 large (macronuclues).

  5. Hematoxylin - eosin stain Balantidiumcoli trophozoite in section of colon

  6. Balantedium coliB. Cyst:Shape round,2 Nuclei micro- and macronuclues.

  7. ( X 1000 ) Iodine stain Balantidiumcoli Trophozoite, stool smear ( 60 – 200 µm ) Trichrome stain Balantidiumcoli Cyst stool smear ( 50 – 60 µm )

  8. Leishmania as a group

  9. Kinetoplastida1. LeishmaniadonovaniA. Amastigote: parasite interacellular in macrophage (Mø); shape round-oval, size 3-4 µm; in burst Møs parasite usually ovoid, 4-6 µm long; nucleus red, kinetoplast red. Amastigotes

  10. Kinetoplastida1. LeishmaniadonovaniA. Amastigote: parasite interacellular in macrophage (Mø); shape round-oval, size 3-4 µm; in burst Møs parasite usually ovoid, 4-6 µm long; nucleus red, kinetoplast red.

  11. LeishmaniadonovaniB. Promastigote: parasite extra-cell shape Spindale- like; nucleus red, central; kinetoplast red, anterior; flagellum anterior. nucleus Kinetoplast Flagellum

  12. Lab: 14 Kinetoplastida 2. Trypanosomes spp.

  13. KINETOPLASTES African trypanosomes Trypanosomagambiense American trypanosome Trypanosomacruzi

  14. 2. Trypanosomagambiense A. Trypamastigote: parasite extra-cellular in blood stream; shape spindale-like, S-shaped; nucleus central;kinetoplast posterior; flagellum arising from posterior end, extending anteriorly along an undulating membrane.

  15. 3.Trypanosoma cruzia. trypamastigot:parasite extra-cellular in blood stream; shape spindale-like, C-shaped; nucleus central; kinetoplast posterior; large, dot-like; flagellum arising from posterior end, extending anteriorly along an undulating membrane.

  16. 3.Trypanosomacruzib. amastigote:parasite intra-celllular; shape round –ovoid; nucleus central;kinetoplast marginal.

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