Isolation and Enrichment of soil bacteria

1. Isolation andEnrichmentofsoilbacteria Regina Gohl, K. Noreen Boyle 02/11/2011 University of Oldenburg - ICBM - PracticalcoursePhysiologyofbacteria (Group B1) – final presentation

2. Regina Gohl, K. Noreen Boyle ICBM Uni Oldenburg 2 Introduction • 1 soil probe  2 experiments Isolation Enrichment ? 37 °C Pasteurisation Nitrogen Nitrogen + yeast Salt Salt+yeast Acidic Alkaline Dilution series Medium variation Introduction Aims & Expectations Courseresults Summary

3. Regina Gohl, K. Noreen Boyle ICBM Uni Oldenburg 3 Aimsandexpectations • Cultivationofsoilbacteria • Determination ofabundance • Comparison: fast vs abundant • Influenceof different media Introduction Aims& Expectations Courseresults Summary

4. Regina Gohl, K. Noreen Boyle ICBM Uni Oldenburg 4 Direct Isolation ~ 10^9 bacteria/g soil 2.8 x 10^6 cells/g soil ~1 % ofbacteriacan becultured in the lab Lessbacteriathanexpected Introduction Aims & Expectations Courseresults Summary

5. Regina Gohl, K. Noreen Boyle ICBM Uni Oldenburg 5 Direct Isolation Introduction Aims & Expectations Courseresults Summary

6. Regina Gohl, K. Noreen Boyle ICBM Uni Oldenburg 6 Enrichment http://www.laborwissen.de/wiki/ tiki-index.php?page=Katalase-Test Introduction Aims & Expectations Courseresults Summary

7. Regina Gohl, K. Noreen Boyle ICBM Uni Oldenburg 7 Enrichment Introduction Aims & Expectations Courseresults Summary

8. Regina Gohl, K. Noreen Boyle ICBM Uni Oldenburg 8 Summary • Resultsasexpected • Varietyofbacteria in soil • Different treatment growth / nogrowth -nitrogensource+yeastextract -nitrogensource Introduction Aims & Expectations Courseresults Summary

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