1 / 14

Mass Spectrometry

Mass Spectrometry. Week 2 Lab S117 Fall 2011. Overview. Post-Lab: Percent Alcohol in Wine TurnItIn.com Working with data sets Analyzing data sets Analysis of experimental procedure Pre-lab: Mass Spectroscopy Fragmentation Protein Analysis Practical matters of lab meeting.

brand
Download Presentation

Mass Spectrometry

An Image/Link below is provided (as is) to download presentation Download Policy: Content on the Website is provided to you AS IS for your information and personal use and may not be sold / licensed / shared on other websites without getting consent from its author. Content is provided to you AS IS for your information and personal use only. Download presentation by click this link. While downloading, if for some reason you are not able to download a presentation, the publisher may have deleted the file from their server. During download, if you can't get a presentation, the file might be deleted by the publisher.

E N D

Presentation Transcript

  1. Mass Spectrometry Week 2 Lab S117 Fall 2011

  2. Overview • Post-Lab: Percent Alcohol in Wine • TurnItIn.com • Working with data sets • Analyzing data sets • Analysis of experimental procedure • Pre-lab: Mass Spectroscopy • Fragmentation • Protein Analysis • Practical matters of lab meeting

  3. Assignments • Sign up for turnitin.com • Formal reports due electronically up to time of lab • One minute24 hours late = 20pt penalty • Give copy of lab notebook to AI in lab

  4. Excel • Working with data sets • Statistical Analysis • Certainty and uncertainty • Measurements • Experimental limit • Reproducibility

  5. Experimental Design • Practical Weaknesses? • Propagation of Error • Was the distillation useful?

  6. Pre-Lab: Mass Spectroscopy Analysis of isotopes!

  7. Nominal Mass and Exact Mass • Nominal Mass: Low resolution • 35Cl = 35 Da • 37Cl = 37 Da • CO = N2 = 28 Da • Exact Mass: High Resolution • CO = 27.9949 Da • N2 = 28.0061 Da

  8. Analysis of Molecules • Parent peak (molecular ion)and fragment peaks • Analysis of fragment patterns useful • Application to Cl2 isovaleraldehyde caffeine

  9. Analysis of Biomolecules • Complex molecules • Molecular mass in kiloDaltons (kDa) • Nobel Prizes for MS of proteins

  10. Proteins Made of Amino Acids

  11. Mass Spec and Mutations • Sickle Cell Anemia • Point Mutation • How can MS be used to identify Mutations?

  12. Identification of Proteins • Comparison of 2D electrophoresis

  13. Identification of Proteins Trypsin Digest MS Library Mass Spec Purified Protein Mix of Peptides Mass Data of mixture Identity of Protein Example 1 ASR LVMK QQK D ASRLVMKQQKD Example 2 ASRLVKMQKQD

  14. Practical Matters • Meet in chemistry computer lab 006 • Mass Spec lab tours • Dry lab: No special clothing requirements! • Remember formal report and lab notebook

More Related