1 / 13

Mass Spectrometry

Mass Spectrometry. WEB resources: http://www.colby.edu/chemistry/OChem/DEMOS/MassSpec.html http://www-methods.ch.cam.ac.uk/meth/ms/theory/index.html. Ionization. Electron. separate m/z. ionize. Fast Atom Bombardment. Plasma Desorption. Ionization. Thermospray. Electrospray. +. -. -.

eto
Download Presentation

Mass Spectrometry

An Image/Link below is provided (as is) to download presentation Download Policy: Content on the Website is provided to you AS IS for your information and personal use and may not be sold / licensed / shared on other websites without getting consent from its author. Content is provided to you AS IS for your information and personal use only. Download presentation by click this link. While downloading, if for some reason you are not able to download a presentation, the publisher may have deleted the file from their server. During download, if you can't get a presentation, the file might be deleted by the publisher.

E N D

Presentation Transcript

  1. Mass Spectrometry WEB resources: http://www.colby.edu/chemistry/OChem/DEMOS/MassSpec.html http://www-methods.ch.cam.ac.uk/meth/ms/theory/index.html Biophysical Tools - Mass Spectrometry

  2. Ionization Electron separate m/z ionize Fast Atom Bombardment Plasma Desorption Biophysical Tools - Mass Spectrometry

  3. Ionization Thermospray Electrospray Biophysical Tools - Mass Spectrometry

  4. + - - + Mass analysis • Quadrupole Mass Filter • 4 parallel rods with mixture of DC and RF electrical voltage • Magnetic sector Force on a moving charge in B field = Centrifugal force: zvB = mv2/r Calculate v from kinetic energy: mv2/2 = z Vacc mass to charge ratio: m/z = r2B2/2Vacc • Electrostatic Analyzer • instead of magnet use electric field: • F = zE Time-of-Flight ions of different m/z and the same energy will have different velocity Biophysical Tools - Mass Spectrometry

  5. Matrix Assisted Laser Desorption accuracy +/- 1 Da on a 10 kDa protein Biophysical Tools - Mass Spectrometry

  6. Ion Cyclotron Resonance • radius of motion is defined by centrifugal force (mv2/r) balancing the magnetic force (zvB) • Angular velocity (cyclotron frequency) defined by m/z ratio Biophysical Tools - Mass Spectrometry

  7. Distribution of charged states in electrospray High resolution neighboring m/z values differing by a single charge: isotope peaks separation: Biophysical Tools - Mass Spectrometry

  8. Combined techniques MS/MS Triple – quadrupole trap Hybrid sector/trap LC/MS Biophysical Tools - Mass Spectrometry

  9. Application: Mass determination Perils of sample preparation Biophysical Tools - Mass Spectrometry

  10. Application: Oligomeric structures Biophysical Tools - Mass Spectrometry

  11. Application: Peptides Biophysical Tools - Mass Spectrometry

  12. Application: Sequencing Biophysical Tools - Mass Spectrometry

  13. Application: postranslational modification Biophysical Tools - Mass Spectrometry

More Related