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RC-LH1 core complex from R. palustris

RC-LH1 core complex from R. palustris. Neil Isaacs Department of Chemistry University of Glasgow. Periplasm. Light. Cytochrome b/c 1. H +. LH2. LH1. e -. e -. H +. Cyt c. Q. Q. B850. B880. e -. Q. RC. Q. Q. Q. B800. Q B. Q. ADP + P i. ATP. H +. H +. Cytoplasm.

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RC-LH1 core complex from R. palustris

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  1. RC-LH1 core complex from R. palustris Neil Isaacs Department of Chemistry University of Glasgow

  2. Periplasm Light Cytochrome b/c1 H+ LH2 LH1 e- e- H+ Cyt c Q Q B850 B880 e- Q RC Q Q Q B800 QB Q ADP + Pi ATP H+ H+ Cytoplasm ATP synthase Photosynthetic system of purple bacteria

  3. Peripheral light-harvesting antenna (LH2 complex) from Rh. Acidophila, McDermott et al. (1995) Nature374, 517.

  4. The Bacterial Reaction Centre First structure of reaction centre, from Rhodopseudomonas viridis, was determined by Deisenhofer, et al. (1985) Nature 318, 618. Shown above is the structure of RC from Rhodobacter sphaeroides, Allen, et al. (1987) PNAS, 84, 5730; McAuley, et al. (1999) PNAS,96, 14706.

  5. Cytochrome bc1(Iwata et al (1998) Science, 281, 64—71)

  6. F1ATPase Abrahams et al, (1994) Nature, 370, 621--8

  7. Periplasm Light Cytochrome b/c1 H+ LH2 LH1 e- e- H+ Cyt c Q Q B850 B880 e- Q RC Q Q Q B800 QB Q ADP + Pi ATP H+ H+ Cytoplasm ATP synthase

  8. Karrasch et al. (1995) EMBO J., 14, 631. Electron Microscopy 8.5 Å projection map for 2D-crystal of the reconstituted light-harvesting complex I (LH1) from Rhodospirillum rubrum.

  9. Hypothetical model of the purple bacteria PSU LH2 RC LH1 Papiz MZ, Prince SM, Hawthornthwaite-Lawles AM, McDermott G, Freer AA, Isaacs NW & Cogdell RJ (1996) Trends Plant Sci. 1, 198-206.

  10. Walz, et al. (1998) J. Mol. Biol.282, 833. Electron Microscopy 25 Å projections for 2D-crystals of the RC-LH1 complex from Rhodobacter sphaeroides.

  11. Diffraction from crystals of the LH1-RC core complex

  12. Current electron density map

  13. QB W

  14. AFM shows the arrangement of photosynthetic complexes in the membrane S. Bahatyrova et al, (2004) Nature, 430, 1058-1062

  15. Questions to tax the brain: How does LH1 interact with RC? How does W interact with RC and LH1? How does the complex assemble? What is protein W? 34 proteins 2 Ubiquinone 19 Bchl 16? Carotenoid 2 Pheophytin Fe > 70 components & no instructions….

  16. Separation of organic solvent soluble polypeptides

  17. LH1-RC core 21 21 b 14 14 a 6.5 6.5 W? 20 a-dimers 15 b-polypeptide 10 a-polypeptide SDS-PAGE of purified low-molecular weight LH polypeptides

  18. The crystal structure of the RC LH1 core complex from Rhodopseudomonas palustris Aleksander W. Roszak, Tina D. Howard, June Southall, Alastair T. Gardiner, Neil W. Isaacs and Richard J. Cogdell BBSRC Wellcome Trust SRS Daresbury, stations14.1 and 9.6

  19. Questions: How does LH1 interact with RC? How does W interact with RC and LH1? How does the complex assemble? What is protein W? 34 proteins 2 Ubiquinone 19 Bchl 16? Carotenoid 2 Pheophytin Fe > 70 components & no instructions….

  20. Cytochrome bc1(Iwata et al (1998) Science, 281, 64—71)

  21. F1ATPase Abrahams et al, (1994) Nature, 370, 621--8

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