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Chapter 8: Cell Reproduction. Review. Organelles Centrioles Made of microtubules Acts as anchors in cell division Mitotic Spindle fibers Two Main types Kinetochore Fibers- Attach from centriole to centromere of chromosomes and assists in movement of chromosomes
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Review • Organelles • Centrioles • Made of microtubules • Acts as anchors in cell division • Mitotic Spindle fibers • Two Main types • Kinetochore Fibers- Attach from centriole to centromere of chromosomes and assists in movement of chromosomes • Polar Fibers – Extend from pole to pole and keep the shape of the cell during cell division • Nuclear envelope • Double Phospholipid bilayer surrounding nucleus • Allows selective passage of RNA and other material • Nucleolus • Dense area where DNA is concentrated in the nucleus
Section 8.1 • Chromosomes • Chromosome Structure • Rod-shaped and made of DNA and proteins called histones • Two full copies of DNA • Form it takes before cell division • Has two identical halves called chromatid • Chromatid • Half of the chromosome • One full copy of DNA • Attached in the center • Centromere • Center where chromatids are held together • Chromatin • Less tightly coiled DNA-protein complex • Tin = thin *Think spaghetti • Form taken during transcription
Section 8.1 • Sex Chromosomes • Determine gender of the organism • May carry genes for other characteristics • Either X or Y • Female = XX Male = XY • One pair (or two chromosomes) • Autosomes • Remaining chromosomes • Contain genes for many traits • 22 pairs (or 44 chromosomes)
Section 8.1 • Homologous Chromosomes • Two copies of each autosome • One copy from each parent • Same size and shape • Carry genes for the same trait • Karyotype • Photomicrograph of chromosomes • Notice there are 22 pairs of autosomes and 1 pair of sex chromosomes • What is the gender of this organism?
Section 8.1 • Diploid • Cells that are diploid contain two autosomes from each homologous pair and two sex chromosomes • Abbreviated as 2n • Memory key * di = two • Occurs in all cells except sperm and egg cells • Haploid • Cells contain only one set of chromosomes • Therefore, half the number of chromosomes of a diploid cell • Abbreviated as 1n • Memory key * hap = half • Sperm cell (1n) and egg cell (1n) create a diploid cell (2n)
Section 8.1 Notice high number of chromosomes in fern compared to humans!
Section 8.1 • Activity • Create a karyotype! • Arrange in pairs according to length, centromere position, and banding pattern • Questions • How many autosomes are there? How many sex chromosomes? • Is the organism a male or female? • Why are karyotypes important tools for geneticists? • Homework • Review questions on p. 153 #1-5
Section 8.1 Review Answers • Name the proteins that DNA wraps around to form a chromosome in eukaryotic cells. • Histones. • How do the structure and location of a prokaryotic chromosome differ from that of a eukaryotic chromosome? • A prokaryotic chromosome consists of a circular DNA molecule. Eukaryotic chromosomes are rod-shaped, associated with histone and nonhistone proteins, and found within the cell’s nucleus. • Does chromosome number indicate whether an organism is a plant or animal? Explain. • No. For example, chimpanzees have the same number of chromosomes as potatoes or plums.
Section 8.1 Review Answers 4. Contrast sex chromosomes with autosomes. • Sex chromosomes determine the gender of an organism. Autosomes are all of the other chromosomes in an organism. 5. Using Table 8-1, list the haploid and diploid number of chromosomes for each organism.
Section 8.2 • Cell Cycle • Repeating set of events in the life of a cell • Interphase • Time between cell divisions • Three phases • G1 – Cell growth • S – DNA is copied • G2 – Growth and prep for cell division • Cell division • Two phases • Mitosis – Nucleus of the cell divides • Cytokinesis – Division of the cell’s cytoplasm
Section 8.2 • Cell Division • Prokaryotes • Remember: Has cell wall, no nuclei, no membrane-bound organelles • Binary fission • Division of prokaryotic cell into two offspring cells
Section 8.2 • Cell Division (cont’d) • Eukaryotes • Mitosis • Division of the nucleus • Four stages (Prophase, Metaphase, Anaphase, Telophase) • Cytokinesis • In animal cells, pinching of cell membrane occurs • Cleavage furrow • In plants, cell plate formation
Section 8.2 • Control of Cell Division • Checkpoints = traffic signal • Three main checkpoints • G1 checkpoint • G2 checkpoint • Mitosis checkpoint
Section 8.2 • Activity • Venn diagram: Cell division in Prokaryotes vs Eukaryotes • Karyotype Part 2 • Homework • Create the cell cycle
Section 8.2 (cont’d) • Mitosis • Prophase • Tight coiling of DNA into chromosomes • Nucleolus and nuclear membrane break down • Centrosomes appear and move to opposite ends of the cell • Kinetochore fibers extend from kinetochore from each chromatid to centrosome • Metaphase = Middle • Kinetochore fibers move chromosomes to center of cell • All chromosomes line up in a single file line
Section 8.2 • Anaphase • Chromosomes separate at the centromere and chromatids move to opposite poles • Chromatids are now considered individual chromosomes • Telophase • Spindle fibers disassemble • Chromosomes return to less tightly coiled chromatin state • Nuclear envelope forms around each set of chromosomes • Nucleolus forms in each of the newly forming cells • Animation
Mitosis vs Meiosis • Where do they occur? • Mitosis – in the body cells • Meiosis – in the germ cells • Why does it occur? • Mitosis • Asexual reproduction • Growth and development • To replace old cells • Meiosis • Formation of gametes (egg and sperm cells) that will be used in sexual reproduction
Mitosis vs Meiosis • End result? 46 46 23 23 46 46 23 23 23 23 MEIOSIS MITOSIS
Meiosis • Also known as reduction division • Occurs in germ cells found in the ovaries and testes • Produces sex cells which are haploid • Occurs in two phases • Meiosis I • Reduces the number of chromosomes from diploid to haploid • Meiosis II • Produces four haploid daughter cells
Meiosis I • Prophase I • DNA coils tightly into chromosomes • Spindle fibers appear • Nucleolus and nuclear envelope disassemble • Synapsis =Pairing of homologous chromosomes • Tetrad =Pair of homologous chromosomes twisted around each other • Crossing over may occur • Genetic recombination
Meiosis I • Metaphase I • Spindle fibers attach to centromere of tetrads • Tetrads line up along midline • Anaphase I • Homologous chromosome pairs are separated and move to opposite poles • Independent assortment occurs • Telophase I • Chromosomes reach opposite ends of cell • Spindle Fibers disappear • Cytokinesis begins
Meiosis II • Prophase II • Spindle fibers form and attach to the centromeres of the double stranded chromosomes • Metaphase II • Chromosomes are moved to the midline of the dividing cell • Anaphase II • Chromatids separate and move toward opposite poles • Telophase II • Nuclear membrane forms in each of the four new cells • Cytokinesis II occurs resulting in four new cells, each with half the original cell’s number of chromosomes
Section 8.3 • Results of mitosis vs. meiosis
Section 8.3 • Development of Gametes • Spermatogenesis • Oogenesis • Polar bodies • Sexual reproduction