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Lecture 1: Introduction and review

Lecture 1: Introduction and review. Quiz 1 Website: http://www.esf.edu/chemistry/nomura/fch530/ Review of acid/base chemistry Universal features of cells on Earth Cell types: Prokaryotes and Eukaryotes

brennan-stephenson
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Lecture 1: Introduction and review

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  1. Lecture 1: Introduction and review • Quiz 1 • Website: http://www.esf.edu/chemistry/nomura/fch530/ • Review of acid/base chemistry • Universal features of cells on Earth • Cell types: Prokaryotes and Eukaryotes • Quiz Friday on the 20 amino acids-you need to know the structures, names, single letter codes, and pKa’s of the side chains

  2. Review of pH, acids, and bases • pH is generally defined as the negative logarithm of the hydrogen ion activities (concentration) expressed over 14 orders of magnitude pH = -log10 [H+] • The pH scale is a reciprocal relationship between [H+] and [OH-] • Because the pH scale is based on negative logarithms, low pH values represent the highest [H+] and thus the lowest [OH-] • At neutrality, pH 7, [H+] = [OH-]

  3. [H+][Cl-] Ka= [HCl] Review of pH, acids, and bases • Strong electrolytes dissociate completely in water • Electrolytes are substances capable of generating ions in solution • Increase the electrical conductivity of the solution • The dissociation of a strong acid in water The equilibrium constant is [H2O] is constant in dilute aq. Solutions and is incorporated into the equilibrium constant. giving rise to a new term Ka-the acid dissociation constant = K[H2O], [H3O+] is expressed as [H+] HCl + H2O H3O+ + Cl- [H3O+][Cl-] K = [H2O][HCl] Because Kais largefor HCl, [H+] in solution = [HCl] added to solution. Thus, a 1M HCl solution has a pH of 0, a 1 mM HCl solution has a pH of 3, and so on. Conversely, 0.1 M NaOH solution has a pH of 13.

  4. [H+][Cl-] Ka= [HCl] For a strong acid Ka will approach be large because the nearly all of the protons will be dissociated. The [H+] at equilibrium is equal to the initial concentration of the acid. Calculate the pH of a 1M HCl solution HCl + H2O H3O+ + Cl- 0.0004% at equilibrium 99.996% at equilibrium Since we are at equilibrium, H3O+ is equal to the initial concentration of acid. [H+] = [H3O+] = [HCl] = 1M We know that pH is the -log of [H+], therefore for 1M HCl at equilibrium pH = -log10 [H+] pH = -log10 (1) pH = 0

  5. [H+] [OH-] pH

  6. Review of pH, acids, and bases CH3COOH + H2O H3O+ + CH3COO- • Weak electrolytes only slightly dissociate in water • Acetic acid, CH3COOH • The dissociation of a weak acid in water The acid dissociation constant is Ka is also called the ionization constant, because Ka is small, most of the acetic acid is not ionized. [H+][CH3COO-] Ka = = 1.74 X 10-5 M [CH3COOH]

  7. HA H+ + A- [H+][A-] Ka = [HA] Acid dissociation constant • The general ionization of an acid is as follows: So the acid dissociation constant is as follows: There are many orders of magnitude spanned by Ka values, so pKa is used instead: pKa = - log10Ka • The larger the value of the pKa, the smaller the extent of dissociation. • pKa <2 is a strong acid

  8. Henderson-Hasselbalch Equation HA H+ + A- • Describes the dissociation of a weak acid in the presence of its conjugate base • The general ionization of a weak acid is as follows: So the acid dissociation constant is as follows: Rearranging this expression in terms of the parameter of interest [H+] gives the following: [H+][A-] Ka = [HA] Ka [HA] [H+] = [A-]

  9. [HA] [HA] [A-] [A-] Henderson-Hasselbalch Equation log Ka + log log[H+] = Take the log of both sides: Change the signs and define pKa as -log Ka : pKa - log pH = or [A-] pKa + log pH = [HA]

  10. x (co-x) vol vol [HA] = ( ) x pKa + log pH = co-x Titration curves and buffers • Titration curves can be calculated by the Henderson-Hasselbalch equation • As OH- is added to the reaction, it reacts completely with HA to form A- x = the equivalents of OH- added and V represents the volume of the solution. If we let co represent HA equivalents initially present, then: We can reincorporate this into the Henderson-Hasselbalch eqn. [A-] =

  11. HA H+ + A- Acid conjugate base [H+][A-] Ka= [HA] Buffers • Buffers are solutions that resist changes in their pH as acid (H+) or base (OH-) is added. • Typically, buffers are composed of a weak acid and its conjugate base. • Acids = Proton (H+) donors • Bases = Proton Acceptors • Acids and their conjugate bases are in equilibrium. Equilibria are related to the properties of the reactants and products, so for weak acids, the tendency to give up its proton determines its buffering property • The tendency to ionize can be put in an equilibrium equation • A solution of a weak acid that has a pH near to its pKa has an equivalent amounts of conjugate base and weak acid. • Typically a weak acid is in its useful buffer range within 1 pH unit of its pKa.

  12. Polyprotic acids • Have more than one acid-base group • H3PO4 and H2CO3 • The pK’s of two closely associated acid-base groups are not independent - the closer they are, the greater the effect. • Examples: oxalic acid and succinic acid O O O O H-O-C-C-O-HH-O-C-CH2CH2-C-O-H pK differs by 3 pH units pK differs by 1.4 pH units

  13. Polyprotic acids • The effect of having successive ionizations from the same center is even greater. • However if pK’s of polyprotic acid differ by less than 2 pH units, this reflects the average ionization of all of the groups.

  14. Universal features of cells • “Life possesses the properties of replication, catalysis, and mutability.” - Norman Horowitz • Life requires free energy. • Main energy currency is • ATP (bond energy, G) • NADH, NADPH (redox energy) • All cells obey the same laws of thermodynamics (see Ch.3). • G (Gibbs free energy) must be negative (spent) • S (Entropy) increases • Sources of energy may vary • Purple sulfur bacteria H2S So • Humans CH2O H2O + CO2 • Plants h

  15. Universal features of cells (cont.) • Most organisms are composed of only 16 chemical elements • (H,C,N,O,P,S,Mn, Fe, Co, Cu, Zn, Na, Mg, Cl, K, Ca). • Chemical makeup appears to be determined partly by the availability of raw materials and the specific roles of molecules in life processes. • Do not reflect the composition of the biosphere • Examples on per atom basis, H in organisms = 49%, H in Earth’s crust = 0.22 %, Si in organisms = 0.033%, Si in Earth’s crust = 28%) • H, O, N, and C, make up >99% by weight of living matter are the smallest atoms that can share 1, 2, 3, and 4 electrons respectively. • O, N, and C are the only elements that easily form strong multiple bonds. • O2 is soluble in water and readily available to all organisms. • Phosphorous and sulfur are unstable in the presence of water. • Require a large amount of energy to form. • Energy released when they are hydrolyzed.

  16. MW N2, H2O, CO2 18-44 5 aromatic bases, ribose 20 amino acids Palmitate, glycerol, choline 100-250 Glucose Amino acids 100-800 Phospholipids Nucleotides Sugars 104-109 Proteins Nucleic acids Polysaccharides Multienzyme complexes, ribosomes, chromosomes, membranes, structural elements 106-1010 Organelles, Cells, Tissues, Organs, Organsims

  17. Universal features of cells (cont.) • All cells function as biochemical factories and use the same basic molecular building blocks. • Proteins (amino acids polypeptides proteins) • Can be structural or catalytic • Enzymes • Transport (Na+/K+ pump) • Storage (ferritin) • Signals (hormones/toxins), examples insulin or botulinum toxin • Receptors • Structure (collagen, elastin) • Lipids (fatty acids lipids) • Membranes • Triglycerides (energy storage) • Phospholipds (membrane structure) • Sphingolipids (found in nerve cells and brain tissue) • Sterols (hormones and membranes)

  18. DNA RNA Protein Universal features of cells (cont.) • Carbohydrates • Monosaccharides (glucose, fructose) • Disaccharides (sucrose, maltose) • Trisaccharides (raffinose) • Complex carbohydryates • Starch (energy storage) • Cellulose (structure, cell wall) • Cell-cell recognition • Nucleic acids • DNA (genetic material) • RNA (mRNA, tRNA, pre-mRNA or hnRNA, rRNA) • Proteins and nucleic acids are produced by the same rules • Central dogma • A living cell can exist with fewer than 500 genes!

  19. Types of cells • There are two major cell types: eukaryotes and prokaryotes. • Eukaryotes have a membrane enclosed nucleus encapsulating their genomic DNA. • Prokaryotes do not have a nucleus. Eukaryotes Fungi, Protists, Animals, Plants 10-100 µm Prokaryotes Bacteria, Archaea 1-10 µm

  20. General schematic for a prokaryote cell

  21. General schematic of an animal cell

  22. General schematic of a plant cell

  23. MW N2, H2O, CO2 18-44 5 aromatic bases, ribose 20 amino acids Palmitate, glycerol, choline 100-250 Glucose Amino acids 100-800 Phospholipids Nucleotides Sugars 104-109 Proteins Nucleic acids Polysaccharides Multienzyme complexes, ribosomes, chromosomes, membranes, structural elements 106-1010 Organelles, Cells, Tissues, Organs, Organsims

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