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Nanoparticle-based Theorem Proving

Nanoparticle-based Theorem Proving. The Target Problem. , add R as. nil. R is true !!. Experimental Section. Preparation of Au nanoparticles Synthesis & Purification of (alkanethiol)-modified oligonucleotides

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Nanoparticle-based Theorem Proving

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  1. Nanoparticle-based Theorem Proving

  2. The Target Problem , add R as nil R is true !!

  3. Experimental Section Preparation of Au nanoparticles Synthesis & Purification of (alkanethiol)-modified oligonucleotides Preparation of 3'- or 5'-(Alkanethiol)oligonucleotide-modified Au nanoparticles Preparation and Characterization of Linker DNA Characterization of DNA-Linked Au Nanoparticle Assembly Transmission Electron Microscopy (TEM) Calculations of UV-vis Spectra

  4. The Oligomer Sequence

  5. UV-vis Spectrum of DNA Linked Au Nanoparticle (A) (B) (C) DNA-linked nanoparticle Assembly

  6. 45 mer 15 mer 15 mer Further Work Selectivity Test Assembly S1 Nuclease Digestion

  7. Surface-based Theorem Proving

  8. 45 mer 15 mer 15 mer Surfaced-based Theorem Proving

  9. (a) ¬RPQ ¬T ¬S N.C P.C R ¬RPQ ¬T N.C P.C ¬S ¬P N.C P.C ¬RPQ ¬T ¬S ST ¬Q (b) ¬RPQ ¬T P.C ¬S N.C ¬RPQ ¬T N.C P.C ¬S N.C P.C ¬RPQ ¬T ¬S • The schematic diagram of theorem proving before (a) and after (b) hybridization. • The immobilized probe DNA sequences on aldehyde-coated slide glass. • Molecular recognition properties by the target molecules between the probe sequences.

  10. Experimental Section Preparation of probe DNA Preparation of Target DNA Hybridization probe & Target DNA Washing Confocal microscope detection

  11. R ¬P ST¬Q 50 pmol 100 pmol Temp. 50°C Constant ST¬Q Temp. 60°C Constant ST¬Q ¬P

  12. R ¬P ST¬Q 50 pmol 100 pmol Temp. 50°C Constant ¬P Temp. 60°C Constant ¬P ¬P

  13. R ¬P ST¬Q 50 pmol 100 pmol Temp. 50°C Constant R Temp. 60°C Constant R ¬P

  14. Discussion • Confocal microscope • 457~633 nm • Detection range dye & Non-overlapping emission wavelength • Eqipment condition is break down now • Microarray scanner (futher exp.) • False positive signal • ¬P and ¬S: non-complementary sequence • Why (?) • GC ratio

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