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Oligonucleotide-based Theorem Proving by Cross-Linking Gold Nanoparticle Assembly

Oligonucleotide-based Theorem Proving by Cross-Linking Gold Nanoparticle Assembly. 2004. 3. 23 Park, Ji-Yoon. Colloidal Gold. Diameter: 5-20 nm SPR (Surface plasmon resonance): 520 nm optical obsorption peak Aggregation (>520 nm: shift the absorption peak)

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Oligonucleotide-based Theorem Proving by Cross-Linking Gold Nanoparticle Assembly

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  1. Oligonucleotide-based Theorem Proving by Cross-Linking Gold Nanoparticle Assembly 2004. 3. 23 Park, Ji-Yoon

  2. Colloidal Gold • Diameter: 5-20 nm • SPR (Surface plasmon resonance): 520 nm optical obsorption peak • Aggregation (>520 nm: shift the absorption peak) • Cross-linking mechanism by target DNA between the gold particle • Application: DNA, lectin, heavy metal ion, potassium ion, protein A

  3. 15 mer 15 mer 45 mer The DNA-based Nanoparticle Assembly Strategy

  4. Purpose • In this paper, we purpose to assembly of DNA-functionalized gold nanoparticles as optical detection approach. • Oligonucleotides offer several advantages over non-biological-based linker molecules. • Various sizes of gold nanoparticles are readily available at low cost, surface modification with probe DNA is easier, and the temperature control for the formation of nanoparticles is not necessary.

  5. The Target Problem , add R as nil R is true !!

  6. Experimental Section Preparation of Au Nanoparticles Synthesis and Purification of (Alkanethiol)-Modified Oligonucleotides Preparation of 3'- or 5'-(Alkanethiol)oligonucleotide-Modified Au Nanoparticles Preparation and Characterization of Linker DNA Preparation and Characterization of DNA-Linked Au Nanoparticle Aggregates Transmission Electron Microscopy (TEM) Calculations of UV-vis Exinction Spectra

  7. The Sequence Information

  8. 520 nm Comparison between Before and After Hybridization

  9. (A) The UV-vis spectrum of oligonucleotide-modified 10-nm particles before (red line) and after (blue line) DNA-induced assembly (B) Solutions of 10-nm-diameter Au particles before (cuvette A) and after (cuvette B) DNA-induced assembly (C) Aqueous solution of 10-nm-diameter Au particles before (red) and after (blue) the addition of NaCl. This is an irreversible process (A) (B) (C)

  10. Concentration Variation of 10 nm Colloidal Gold

  11. Conclusions • A new “programmed assembly” strategy for organizing nanoparticles into periodic functional materials • The strategy utilize nanoparticle building blocks modified with alkanethiol-capped oligonucleotides and complementary linker oligonucleotide strands to form extended structures with control over particle chemical composition, periodicity, and aggregated thermal stability • Highly selective diagnostic method relies on the distance-dependent optical properties of gold particles

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