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Systems Biology Project

Systems Biology Project . 1,3-Propanediol . Group2 生科 陳麗如 生科 林嘉慧 資工 陳奎昊 資工 劉盈詮 . Introduction of 1,3-Propanediol. The usage of 1,3-Propanediol. As a monomer in the synthesis of polymethylene terephthalates and the manufacture of polyurethanes and cyclic compounds.

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Systems Biology Project

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  1. Systems Biology Project 1,3-Propanediol Group2 生科 陳麗如 生科 林嘉慧 資工 陳奎昊 資工 劉盈詮

  2. Introduction of 1,3-Propanediol

  3. The usage of 1,3-Propanediol As a monomer in the synthesis of polymethylene terephthalates and the manufacture of polyurethanes and cyclic compounds. Important intermediate for synthetic, it can be used as intermediate of Vitamin H, PTT, 1,3 Dibrominepropane, 1, 3-Propanediol methyl ether, 3-chloro-1-propanol and so on. As a material for the manufacture of carpeting and textile fibers 特性: 柔軟,伸展及回復, 容易照料, 耐髒及固色等特性的布料. (含PDO的聚合物結構中有一種獨特的糾結使其於伸展時可快速地恢復, 此特性使得布料 (例如在膝蓋及手肘處)於伸展時可快速回復到原來的狀態.

  4. Find the metabolic pathways involved in the synthesis of 1,3-Propanediol 1,3-propanediol dehydrogenase 3-hydroxypropanal NAD+ NADH

  5. Find the enzymes and genes involved in these pathway reactions 2 1 3 4 - - 5 glycerol-3-phosphate dehydrogenase (NAD+) triosephosphate isomerase (TIM) glycerol-1-phosphate phosphohydrolase glycerol dehydratase 1,3-propanediol dehydrogenase

  6. Triosephosphate isomerase (TIM), E5.3.1.1, tpiA

  7. glycerol-3-phosphate dehydrogenase (NAD+), E1.1.1.8, GPD1 HSA: 2819(GPD1)MMU: 14555(Gpd1)RNO: 60666(Gpd3)DME: CG3215-PA(CG3215) CG9042-PA(CG9042) CG9042-PB(CG9042)CG9042-PC(CG9042)CEL: F47G4.3K11H3.1aATH: At5g40610(MNF13.130)CME: CMD113CSCE: YDL022W(GPD1) YOL059W(GPD2)AGO: ADR311C(ADR311Cp)CAL: orf19.1756(GPD2) orf19.691(GPD1)SPO: SPAC23D3.04c(gpd2) SPBC215.05(gpd1)ECU: ECU05_0270PFA: PF11_0157PFL0780wCHO: Chro.20028WOL: WD0731(gpsA)

  8. glycerol dehydratase, E4.2.1.30 STY: STY2245 (pduC) STY2246 (pduD) STY2247 (pduE)STT: t0832 (pduE) t0833 (pduD) t0834 (pduC)STM: STM2040 (pduC) STM2041 (pduD) STM2042 (pduE)MLO: mll6722LMO: lmo1153lmo1154lmo1155LMF: LMOf2365_1161 (pduC) LMOf2365_1162 (pduD) LMOf2365_1163 (pduE)LIN: lin1117lin1118lin1119CPE: CPE0929CPE0930CPE0931

  9. 1,3-propanediol dehydrogenase, E1.1.1.202 BCE: BC2176LPL: lp_3051 (dhaT)CTC: CTC00506LIL: LA2996 (dhaT)LIC: LIC11070AAE: aq_1145 (dhaT)

  10. Co-factor manipulation Coenzyme B12-inact glycerol dehydratase Coenzyme B12-dependent enzyme reactivase Coenzyme B12-inact glycerol dehydratase Coenzyme B12 glycerol dehydratase

  11. Phosphotransferase system (PTS) Tpi: Triosephosphate isomerase DAR1: glycerol 3-phophate dehydrogenase GPP2: glycerol 3-phophate phosphatase DhaB: glycerol dehydratase DhaT: 1,3-propenediol dehydratase

  12. acrolein Ethylene oxide H2O, H2 CO2, H2 energy-intensive and required expensive starting materials Shell process Degussa/Dupont process H2 1,3-propanediol Dupont/Genencor process Yeast/ E. coli Two-stage production glucose glucose glycerol PDO Yeast E. coli

  13. Is E. Coli suitable for biosynthesis of 1,3-Propanediol Yes! Reason : Well-studied organism Suitable for genetic engineering Large-scale production (production rate, life cycle…) Goal: develop one step system for 1,3-propanediol production Our Strategy: Search for a most suitable E. coli strain ex. K12 Key genes co-express ex. In the same operon Promoter induced by glucose ex. (COX)-2, GLP-1 , SGLT1 Glucose-induced promoter

  14. PDO production (cont.)

  15. Thanks for your attention

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