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Polymerase Chain Reactions: Denaturation, annealing & elongation

Polymerase Chain Reactions: Denaturation, annealing & elongation. 95 o C 50-65 o C 72 o C Polymerase is thermophilic can withstand high temperature. Exponential growth. Generally take through 20-30 cycles 2(doubling with every cycle) 20-30(cycles) 2 20 = 10 6 copies 2 30 =10 9 copies.

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Polymerase Chain Reactions: Denaturation, annealing & elongation

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  1. Polymerase Chain Reactions:Denaturation, annealing & elongation • 95oC • 50-65oC • 72oC • Polymerase is thermophilic can withstand high temperature

  2. Exponential growth • Generally take through 20-30 cycles • 2(doubling with every cycle)20-30(cycles) • 220 = 106 copies • 230 =109 copies

  3. Cycle Sequencing:Denaturation, annealing & elongation • 95oC • 50-65oC • 72oC • Polymerase is thermophilic can withstand high temperature

  4. Exponential growth No exponential growth thus need fluoro labels to see

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