1 / 1

Exercises Protomics I

Exercises Protomics I. Exercise 1

bryce
Download Presentation

Exercises Protomics I

An Image/Link below is provided (as is) to download presentation Download Policy: Content on the Website is provided to you AS IS for your information and personal use and may not be sold / licensed / shared on other websites without getting consent from its author. Content is provided to you AS IS for your information and personal use only. Download presentation by click this link. While downloading, if for some reason you are not able to download a presentation, the publisher may have deleted the file from their server. During download, if you can't get a presentation, the file might be deleted by the publisher.

E N D

Presentation Transcript

  1. ExercisesProtomics I Exercise 1 Searchthe ArrayExpress database (orsimilar) forthree genes withspecificexpression in kidneyandthreeother genes withskin-specificexpression. Ifpossible, selectforeachtissueoneexamplewithhighexpressionlevel, onewith intermediate levelandonewithlowexpressionlevel. Go totheUniprotdatabaseandsearchtheaccessionnumbersforthe 6 genes. Also write down thecalculatedmolecularweightforeachprotein. Look upthesix genes in the Human Proteome Atlas. Do theproteinsshowthe same expressionpropertiesasthemRNAs? Can youidentify in whatcelltypestheproteinsareexpressed? Save representativefigures UsetheProteome Atlas tolookupthesubcellularlocalizationandnotetheresultsandrepresentativefigures. Check thespecificityoftheantibodies. Do theproteinexpressiondata (histology, subcellular) rely on onlyoneantibody? Doesthisantibodyrecognize a single band oftheexpectedmolecularweight? Iftherearedifferences, cantheybeexplained? Exercise2 Thereis a publiclyaccessibledatabaseforproteinabundance in multipeorganisms (and multiple tissuesforsomeofthem). Try to find the web pageofthedatabase. Query thedatabaseforthe top 5 most abundant proteins in human bloodplasmaandbloodserum. Are these different genes? Whatisthedifferencebetween Plasma and Serum anyway? Query the ArrayExpress database (orBioGPS) toseewherethemRNAsofthese genes areexpressed. Whatarethe top 5 most abundant genes in brainandlung? Are theseresultsrealistic? (UseUniprotorWikipediato find out more on the genes ifnecessary) In thelistofhighlyexpressedbrainproteins, identifyonegenewith neuronal expressionandonewithglialexpression. Check the Human Proteomeatlasifthesedifferencesarevisible in theimmunohistologyslides.

More Related