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Staining and Observation of Microorganisms

Exercise 10Objective: To learn proper smear technique1st Step in good staining is preparation of a good smearGoals of smearing:To adhere cells to slide (so they are not washed away in staining procedures)To insure that shrinkage of cells does not occurTo prepare thin smearsMethods:Smears may be prepared from liquid or solid media (See pg. 88 of lab manual)Air driedHeat fixed (kills cells)Always use aseptic technique when preparing smears.

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Staining and Observation of Microorganisms

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    1. Staining and Observation of Microorganisms

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