1 / 3

The Jackson Laboratory Bar Harbor, ME

Genetic tools and resources for orofacial clefting research. PIs: Leah Rae Donahue, Ph.D. Stephen Murray, Ph.D. Co-Investigators: Tom Gridley, Ph.D. The Jackson Laboratory Bar Harbor, ME. FaceBase Mouse Repository at JAX importation, cryopreservation, GQC, distribution.

bryson
Download Presentation

The Jackson Laboratory Bar Harbor, ME

An Image/Link below is provided (as is) to download presentation Download Policy: Content on the Website is provided to you AS IS for your information and personal use and may not be sold / licensed / shared on other websites without getting consent from its author. Content is provided to you AS IS for your information and personal use only. Download presentation by click this link. While downloading, if for some reason you are not able to download a presentation, the publisher may have deleted the file from their server. During download, if you can't get a presentation, the file might be deleted by the publisher.

E N D

Presentation Transcript

  1. Genetic tools and resources for orofacialclefting research PIs: Leah Rae Donahue, Ph.D. Stephen Murray, Ph.D. Co-Investigators: Tom Gridley, Ph.D. The Jackson LaboratoryBar Harbor, ME

  2. FaceBase Mouse Repository at JAX importation, cryopreservation, GQC, distribution • ~ 50 strains in the JAX FaceBase Repository • most are public, some still private, some imported and under development for distribution • new knockout strains - most on B6;129 background • Bambi, Osr1, Osr2, Jag2, Snai1 and Snai2, Gad1, Slc32a1, Tgfbr2, Wnt9b • new Cre strains • Osr2 (B6.129S1), Tgfb3 (STOCK) • new ENU induced mutant strain (on FVB.A) • Irf6 Genetic Resource Science

  3. FaceBaseCre Driver Project:Current progress and future plans • Goal: To generate 15 Cre driver strains for orofacialclefting research • Initial plan is to make four BAC transgenics (Krt6-Cre, Tbx22-CreERT2, dNp63-CreERT2, Lhx7/8-Cre) and three enhancer (Visel project) CreERT2 lines (R26 knock-in). • Current progress: • All basic cassettes (CreIRESmCherry, ROSA targeting vector, etc) are complete • 2 BAC Cres (Tbx22 and p63) are cloned, Krt6 is nearly done (unique challenges). Injections to be scheduled • 2 enhancer elements are ready for targeting into JM8A3 ES cells (this month) • Future enhancer cloning will be very simple and efficient • On track to have first 7 Cre lines made by 9/2010 and characterized by 12/2010 • Future plans • Continue to develop approach as better technology emerges (e.g. 3’ UTR knock-in): proof of principle using same promoter? • Choose new drivers from data generated in other FaceBase projects and in consultation with mouse subcommittee members

More Related