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PROTEIN SYNTHESIS

This article explores the process of protein synthesis, including the role of DNA, genes, and RNA. It covers topics such as the structure of DNA, the role of proteins in building cells, the different types of RNA, and the pathway to making a protein.

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PROTEIN SYNTHESIS

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  1. PROTEIN SYNTHESIS

  2. DNA and Genes

  3. DNA • DNA contains genes, sequences of nucleotide bases • These Genes code for polypeptides (proteins) • Proteins are used to build cells and do much of the work inside cells

  4. Genes & Proteins • Proteins are made of amino acids linked together by peptide bonds • 20 different amino acids exist

  5. Amino Acid Structure

  6. Polypeptides • Amino acid chains are called polypeptides

  7. DNA Begins the Process • DNA is found inside the nucleus • Proteins, however, are made in the cytoplasm of cells by organelles called ribosomes • Ribosomes may be free in the cytosol or attached to the surface of rough ER

  8. Starting with DNA • DNA ‘s code must be copied and taken to the cytosol • In the cytoplasm, this code must be read so amino acids can be assembled to make polypeptides (proteins) • This process is called PROTEIN SYNTHESIS

  9. What macromolecule builds cells? • Lipids • Proteins • Carbohydrates • Nucleic Acids 20

  10. What are proteins made of? • DNA • RNA • Amino Acids • Nucleic Acids 20

  11. How many amino acids exist? • 10 • 20 • 30 • 46 20

  12. A chain of amino acids is a: • Protein • Polypeptide • Both A & B • None of the Above 20

  13. Where are proteins made? • Cytosol • Mitochondria • ER • Ribosomes • Nucleus 20

  14. RNA

  15. Roles of RNA and DNA • DNA is the MASTER PLAN • RNA is the BLUEPRINT of the Master Plan

  16. RNA Differs from DNA • RNA has a sugar ribose DNA has a sugar deoxyribose

  17. Other Differences • RNA contains the base uracil (U) DNA has thymine (T) • RNA molecule is single-stranded DNA is double-stranded DNA

  18. Structure of RNA

  19. . Three Types of RNA • Messenger RNA (mRNA) copies DNA’s code & carries the genetic information to the ribosomes • Ribosomal RNA (rRNA), along with protein, makes up the ribosomes • Transfer RNA (tRNA) transfers amino acids to the ribosomes where proteins are synthesized

  20. Messenger RNA • Long Straight chain of Nucleotides • Made in the Nucleus • Copies DNA & leaves through nuclear pores • Contains the Nitrogen Bases A, G, C, U ( no T )

  21. Messenger RNA (mRNA) • Carries the information for a specific protein • Made up of 500 to 1000 nucleotides long • Sequence of 3 bases called codon • AUG – methionine or start codon • UAA, UAG, or UGA – stop codons

  22. Ribosomal RNA (rRNA) • rRNA is a single strand 100 to 3000 nucleotides long • Globular in shape • Made inside the nucleus of a cell • Associates with proteins to form ribosomes • Site of protein Synthesis

  23. The Genetic Code • A codon designates an amino acid • An amino acid may have more than one codon • There are 20 amino acids, but 64 possible codons • Some codons tell the ribosome to stop translating

  24. The Genetic Code • Use the code by reading from the center to the outside • Example: AUG codes for Methionine

  25. Name the Amino Acids • GGG? • UCA? • CAU? • GCA? • AAA?

  26. Remember the Complementary Bases On DNA: A-T C-G On RNA: A-U C-G

  27. Transfer RNA (tRNA) • Clover-leaf shape • Single stranded molecule with attachment site at one end for an amino acid • Opposite end has three nucleotide bases called the anticodon

  28. amino acid attachment site U A C anticodon Transfer RNA

  29. Codons and Anticodons • The 3 bases of an anticodon are complementary to the 3 bases of a codon • Example: Codon ACU Anticodon UGA UGA ACU

  30. What sugar is found in RNA? • Glucose • Sucrose • Ribose • Deoxyribose 20

  31. What are the 4 nitrogenous bases in RNA? • A G C T • A G C U • A G T U • G C T U 20

  32. DNA has __ strand(s), RNA has ____ strand(s). • 2 , 2 • 2 , 3 • 1 , 2 • 2 , 1 20

  33. The type of RNA that transfers amino acids to ribosomes. • snRNA • mRNA • rRNA • tRNA 20

  34. The tyoe of RNA that makes up the ribosome. • snRNA • mRNA • rRNA • tRNA 20

  35. The type of RNA that copies DNA’s code. • snRNA • mRNA • rRNA • tRNA 20

  36. What is a sequence of three nitrogen bases called? • DNA • RNA • Codon • Antiparrallel 20

  37. What mRNA would be made from this DNA sequence: ATCGGCTAA • TAGCCGATT • ATCGGCTAA • UAGCCGAUU • TUGCCGUTT 20

  38. Transcription and Translation

  39. Pathway to Making a Protein DNA mRNA tRNA (ribosomes) Protein

  40. Protein Synthesis • The production or synthesis of polypeptide chains (proteins) • Two phases:Transcription & Translation • mRNA must be processed before it leaves the nucleus of eukaryotic cells

  41. Nuclear membrane DNA Transcription Pre-mRNA RNA Processing mRNA Ribosome Translation Protein DNA RNAProtein Eukaryotic Cell

  42. Transcription • The process of copying the sequence of one strand of DNA, the template strand • mRNA copies the template strand • Requires the enzyme RNA Polymerase

  43. Template Strand

  44. Question: • What would be the complementary RNA strand for the following DNA sequence? DNA 5’-GCGTATG-3’

  45. Answer: • DNA 5’-GCGTATG-3’ • RNA 3’-CGCAUAC-5’

  46. Transcription • During transcription, RNA polymerase binds to DNA and separates the DNA strands • RNA Polymerase then uses one strand of DNA as a template to assemble nucleotides into RNA

  47. Transcription • Promoters are regions on DNA that show where RNA Polymerase must bind to begin the Transcription of RNA • Called the TATA box • Specific base sequences act as signals to stop • Called the termination signal

  48. RNA Polymerase

  49. mRNA Processing • After the DNA is transcribed into RNA, editing must be done to the nucleotide chain to make the RNA functional • Introns, non-functional segments of DNA are snipped out of the chain

  50. mRNA Editing • Exons, segments of DNA that code for proteins, are then rejoined by the enzyme ligase • A guanine triphosphate cap is added to the 5” end of the newly copied mRNA • A poly A tail is added to the 3’ end of the RNA • The newly processed mRNA can then leave the nucleus

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