Overview of Our Integrated Proteomics and Bioinformatics Services Platform

1. Overview of Our Integrated Proteomics and Bioinformatics Services Platform The Systems Proteomics Company 2011 March 25

2. The Kinexus Discovery Platform KINEX MICROARRAY SERVICES KINETWORKS BLOTTING SERVICES MASS SPEC SERVICES PROBE PRODUCTION SERVICES IN VIVO SERVICES IN SILICO SERVICES • We have created a highly integrated platform of unique proteomics and bioinformatics services to identify disease biomarkers and drug targets and to foster signal transduction research • Our proteomics services are unmatched by any company for convenience, breadth, accuracy, cost and turn-around time GRAPHICS SERVICES IMMUNO- HISTOCHEM 2

3. Kinexus offers cell lysates from 16 of the most commonly studied human tumour cell lines prepared according to client specifications for proteomics analysis 12 cell lines 16 cell lines KINEX MICROARRAY SERVICES KINETWORKS BLOTTING SERVICES MASS SPEC SERVICES PROBE PRODUCTION SERVICES IN VIVO SERVICES IN SILICO SERVICES Services CELL CULTURE 3

4. Clients can also choose from over 230 pre-made cell and tissue lysates for use in our proteomics services >40 monkey, rat + mouse tissues PROTEIN CHARACTERIZATION ANIMAL TISSUES KINEX MICROARRAY SERVICES KINETWORKS BLOTTING SERVICES MASS SPEC SERVICES PROBE PRODUCTION SERVICES IN VIVO SERVICES IN SILICO SERVICES Services ANTIBODY VALIDATION 12 cell lines 16 cell lines CELL CULTURE 4

5. >800 antibodies >650 antibodies Close up of 1 of 16 grids on a Kinex™ Antibody Microarray - control (blue) and treated (red) have been overlaid and staggered for comparison. KINEX MICROARRAY SERVICES KINETWORKS BLOTTING SERVICES MASS SPEC SERVICES PROBE PRODUCTION SERVICES IN VIVO SERVICES IN SILICO SERVICES Microarray Services ANTIBODY MICROARRAYS 5

6. >230 lysates Close up of a section of the Standard Kinex™ Reverse Lysate Microarray. Custom KRLM chips can also be produced for our clients with their own cell/tissue lysates. KINEX MICROARRAY SERVICES KINETWORKS BLOTTING SERVICES MASS SPEC SERVICES PROBE PRODUCTION SERVICES IN VIVO SERVICES IN SILICO SERVICES Microarray Services ANTIBODY MICROARRAYS >800 antibodies >650 antibodies REVERSE MICROARRAYS 6

7. ~200 kinases Close up of a section of the Kinex™ Protein Kinase Microarray. The binding of an ATP analogue to active protein kinase is shown. KINEX MICROARRAY SERVICES KINETWORKS BLOTTING SERVICES MASS SPEC SERVICES PROBE PRODUCTION SERVICES IN VIVO SERVICES IN SILICO SERVICES Microarray Services ANTIBODY MICROARRAYS >800 antibodies >650 antibodies REVERSE MICROARRAYS >230 lysates PROTEIN KINASE MICROARRAYS 7

8. Close up of a section of the Kinex™ Kinase Substrate Microarray chip. The binding of a phosphate detection dye to phosphorylated peptides is shown. ~450 peptides KINEX MICROARRAY SERVICES KINETWORKS BLOTTING SERVICES MASS SPEC SERVICES PROBE PRODUCTION SERVICES IN VIVO SERVICES IN SILICO SERVICES Microarray Services ANTIBODY MICROARRAYS >800 antibodies >650 antibodies REVERSE MICROARRAYS >230 lysates PROTEIN KINASE MICROARRAYS ~200 kinases PEPTIDE MICROARRAYS 8

9. 2 screens KINEX MICROARRAY SERVICES KINETWORKS BLOTTING SERVICES MASS SPEC SERVICES PROBE PRODUCTION SERVICES IN VIVO SERVICES IN SILICO SERVICES KPKS-1.2 multi-immunoblot showing half of the protein kinases detected with this screen. Multi-Immunoblotting Services Kinases Phosphatases PAN-SPECIFIC AB 9

10. 4 screens Overlay of two KPSS multi-immunoblots showing the effects of staurosporin on Jurkat T cell phosphorylation. Red bands correspond to increased phosphorylation and blue bands reveal decreases in phosphorylation. KINEX MICROARRAY SERVICES KINETWORKS BLOTTING SERVICES MASS SPEC SERVICES PROBE PRODUCTION SERVICES IN VIVO SERVICES IN SILICO SERVICES Multi-Immunoblotting Services Kinases Phosphatases 2 screens PAN-SPECIFIC AB PHOSPHO- SPECIFIC AB Phosphosites 10

11. 2 screens • KCPS 1.0 - 18 antibodies tested on 1 lysate sample • KCSS 1.0 - 8 cell/tissue samples tested with up to 3 antibodies KINEX MICROARRAY SERVICES KINETWORKS BLOTTING SERVICES MASS SPEC SERVICES PROBE PRODUCTION SERVICES IN VIVO SERVICES IN SILICO SERVICES Multi-Immunoblotting Services Kinases Phosphatases 2 screens PAN-SPECIFIC AB PHOSPHO- SPECIFIC AB Phosphosites 4 screens CUSTOM AB 11

12. Identify cross- reactive proteins PROTEIN ID Track hundreds of phosphosites PROTEOME PHOSPHORYLATION Identify phospho- sites in a protein PROTEIN PHOSPHORYLATION Identify kinase Substrate proteins KINASE SUBSTRATE PROFILING KINEX MICROARRAY SERVICES KINETWORKS BLOTTING SERVICES MASS SPEC SERVICES PROBE PRODUCTION SERVICES IN VIVO SERVICES IN SILICO SERVICES Mass Spectrometry Services 12

13. Obtain a specificity matrix for a kinase KINASE SPECIFICITY PREDICTION Identify kinases for a phosphosite KINASE MATCH PREDICTION Identify substrates of a kinase PHOSPHOPROTEIN MATCH PREDICTION Identify kinases for many phosphosites KINEX MICROARRAY SERVICES KINETWORKS BLOTTING SERVICES MASS SPEC SERVICES PROBE PRODUCTION SERVICES IN VIVO SERVICES IN SILICO SERVICES In Silico Prediction Services 13

14. Active enzymes and substrates RECOMBINANT PROTEINS SYNTHETIC PEPTIDES Soluble peptides Peptides on macroarrays PEPTIDE ARRAYS Peptides on microarrays KINEX MICROARRAY SERVICES KINETWORKS BLOTTING SERVICES MASS SPEC SERVICES PROBE PRODUCTION SERVICES IN VIVO SERVICES IN SILICO SERVICES Probe Production Services 14

15. Antibody-driven Biomarker Discovery In Vivo custom cell culture and treatment Kinex™ Antibody Microarrays Kinetworks™ Multi-immunoblotting Kinex™ Reverse Lysate Microarrays IHC Immunohistochemistry KiNET and SigNET websites PREPARATION DISCOVERY VALIDATION VALIDATION LOCATION CORRELATION 15

16. Protein Kinase Drugs • More than 30% of world pharmaceutical research is focused on protein kinases • Over 400 human diseases linked to defects in kinase signalling • 19 drugs approved and > 150 in clinical trials • Most kinase inhibitors in clinical development are for cancer treatment EGF receptor 3D structure with kinase inhibitor drug Tarceva 16

17. Kinexus Discovery Platform Assists All Phases of Drug Discovery and Development DRUG TARGET DISCOVERY DRUG TARGET VALIDATION DRUG DISCOVERY DRUG ANALYSIS PROTEIN SCREENING CELL/TISSUE SCREENING HTP COMPOUND SCREENING CELLS MECHANISM PROBE PRODUCTION IN SILICO MICROARRAYS MULTI-BLOT MASS SPEC IN VIVO ANIMALS TOXICOLOGY HUMAN PHASE I, II + III TRIALS PATIENT SCREENING PRE-CLINICAL VALIDATION CLINICAL VALIDATION BIOMARKER DISCOVERY 17

18. Kinex™ KAM Kinetworks™ In Silico Prediction KiNET DB SigNET KB In Silico - IKSP Kinex™ - KKSM Peptide Production Kinase Testing - IKPT In Silico - IKMP Kinex™ KAM - CKSP Mass Spec - MSKS Kinex™ KPKM Binding Assays - Kinex™ - KPKM Activity Assays - KICP Kinexus Services for Kinase Drug Discovery DRUG TARGET KINASE DISCOVERY KINASE SUBSTRATE DISCOVERY KINASE ASSAY DEVELOPMENT KINASE DRUG SPECIFICITY PROTEIN SCREENING CELL/TISSUE SCREENING HTP COMPOUND SCREENING CELLS MECHANISM 18

19. KiNET DataBase Online at www.kinet.ca • Kinetworks™ Immunoblotting DataBase • First launched in 2006 • Features results from >6,000 immunoblots • Over 200,000 protein measurements • Search by protein, treatment or cell/tissue • Over 95% unpublished data KiNET DATABANK 19

20. SigNET KnowledgeBank Online at www.phosphonet.ca • PhosphoNET KnowledgeBase • Free, online access • >92,000 confirmed and 560,000 predicted human phosphorylation sites • >23,000 human proteins • Functional information for ~2% of phosphosites • Evolutionary analyses • Kinase prediction SigNET KNOWLEDGE- BANK 20

21. www.kinexus.ca ADVISORY BOARD Kinexus Bioinformatics Corp. Suite 1, 8755 Ash Street Vancouver, B.C. Canada V6P 6T3 Phone: 1-604-323-2547 Facsimile: 1-604-323-2548 E-mail: info@kinexus.ca 21