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1. Absorbance (600 nM). 0.1. 0.01. 100. 25. 50. 75. 0. Time (h). Figure S2. Effect of the loss of ptsHIcrr operon function on L-ascorbate utilization. The two strains were JM101 (wild type, u ) and PB11 ( ∆ptsHI
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1 Absorbance (600 nM) 0.1 0.01 100 25 50 75 0 Time (h) Figure S2. Effect of the loss of ptsHIcrr operon function on L-ascorbate utilization. The two strains were JM101 (wild type, u) and PB11 (∆ptsHI crr, n). Growth was conducted as described in Figure 2. The addition of cyclic AMP (0.25, 0.5, 1.0 or 2.5 mM; all other symbols) to the medium did not promote detectable growth. Concentrations of cyclic AMP up to 1.0 mM did not inhibit growth of the wild-type strain and 2.5 mM cyclic AMP inhibited only 30%.